Preparation and cultivation methods of fluid nutrient medium of stichopus japonicus in-vivo parasitic deuterostomia

A liquid culture medium and parasitic technology, applied in animal husbandry and other fields, can solve the problems such as the preparation and cultivation method of liquid culture medium in vitro and the difficulty in realizing long-term living culture of the parasitic ostomum in sea cucumbers, etc. Achieve the effects of improving survival rate and survival time, simple preparation method and low cost

Active Publication Date: 2013-02-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the unique habit of the worm parasitizing the respiratory organs of animals, it is difficult to achieve long-term live culture of the worm in vitro by using the conventional method of crushing the animal tissue to prepare the culture medium
[0005] Before the formation of the present invention, reports on A. japonicus ciliates at home and abroad mainly focused on epidemiology, pathogen taxonomy and histopathology, etc., but did not retrieve the in vitro liquid of A. japonicus internal parasitic Detailed report on culture medium preparation and culture method

Method used

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  • Preparation and cultivation methods of fluid nutrient medium of stichopus japonicus in-vivo parasitic deuterostomia
  • Preparation and cultivation methods of fluid nutrient medium of stichopus japonicus in-vivo parasitic deuterostomia

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Embodiment 1

[0020] A kind of Apostichopus in vivo parasitic deuterostomum liquid culture medium is prepared, and concrete steps are as follows: add ethylenediaminetetraacetic acid (EDTA) in seawater, the final concentration of EDTA is 10 -7 mol / L, then boil and sterilize seawater with EDTA added for 15 minutes, cool to 3°C and add fresh sea cucumber body cavity fluid, the volume ratio of body cavity fluid and sterilized seawater is: 1:500. The prepared medium can be stored at 4°C for 2 days for use.

[0021] In November 2010, ostomiasis occurred in sea cucumbers from a sea cucumber farm in Jiaonan, Shandong Province. The diseased sea cucumbers were placed in ice-filled foam boxes and transported back to the Yellow Sea Fisheries Research Institute (Qingdao), China Academy of Fishery Sciences for further investigation. In vitro culture of worms.

[0022] Use sterilized seawater cooled to 4°C to wash the respiratory tree tissue with A. japonicus A. japonicus into a sterilized glass petri di...

Embodiment 2

[0026] A method for preparing a liquid culture medium for parasitic deuterostomes in sea cucumbers, the specific steps are as follows: add ethylenediaminetetraacetic acid (EDTA) to seawater, and the final concentration of EDTA is 10 -5 mol / L, and then boil and sterilize the EDTA-added seawater for 30 minutes, cool to 10°C and add fresh sea cucumber coelom fluid, the volume ratio of coelom fluid and sterilized seawater is 1:1000. The prepared medium can be stored at 4°C for 2 days for use.

[0027] In October 2011, ostomiasis occurred in sea cucumbers from a sea cucumber farm in Penglai, Shandong. The diseased sea cucumbers were placed in ice-filled foam boxes and transported back to the Yellow Sea Fisheries Research Institute (Qingdao), China Academy of Fishery Sciences for further investigation. In vitro culture of worms.

[0028] Use sterilized seawater cooled to 4°C to wash the respiratory tree tissue with A. japonicus A. japonicus into a sterilized glass petri dish; draw ...

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Abstract

The invention discloses a preparation method of a fluid nutrient medium of stichopus japonicus in-vivo parasitic deuterostomia, and belongs to the field of disease prevention and control of mariculture animals. The preparation method comprises the following steps of: adding EDTA (ethylene diamine tetraacetic acid) with the final concentration of 10<-7>-10<-5>mol/L into seawater; boiling and sterilizing the seawater for 15-30min, wherein the EDTA is added into the seawater; and cooling to 3-10 DEG C, and then adding fresh stichopus japonicus body cavity liquid, wherein the volume ratio of the body cavity liquid to the sterilized seawater is equal to 1:500 to 1:1000. By using the preparation method, the in-vitro cultivation time of the stichopus japonicus in-vivo parasitic deuterostomia in the nutrient medium can be up to 60 days, and the technical problems of short in-vitro survival time of the deuterostomia, difficultly in studying the life history of the deuterostomia and difficultly in choosing prevention and treatment medicines are solved.

Description

technical field [0001] The invention belongs to the field of disease prevention and control of seawater cultured animals, and in particular relates to a method for preparing and cultivating a liquid culture medium for parasitic deuterostomum in sea cucumbers. Background technique [0002] Sea cucumber (Apostichopus japonicus) is the species with the highest economic value among sea cucumbers in my country, and its geographical distribution is my country's Liaodong Peninsula and Shandong Peninsula, eastern Russia, Japan and the coast of Korea. In 2010, the national sea cucumber culture area increased by about 150,000 hectares, with an output of 130,000 tons and an output value of more than 20 billion yuan. It has become one of the species with the highest output value of a single species of mariculture in my country, and plays a pivotal role in the coastal fishery economy. [0003] However, with the expansion of breeding scale, sea cucumber diseases occur frequently, causing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 荣小军李彬廖梅杰王印庚王岚
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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