Feed additive for improving DHA conversion efficiency and content in poultry egg, and application thereof
A feed additive and a technology for the conversion rate of docosahexaenoic acid, which is applied in the fields of application, animal feed, animal feed, etc., can solve problems that have not been seen, and achieve the effects of reducing EPA content, increasing DHA content, and improving efficiency
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Embodiment 1
[0032] Preparation and application of embodiment 1 feed additive
[0033] 1, the preparation of flax leaf alcohol extract
[0034] Get 1000g of fresh flax leaves, wrap them in filter paper, and extract them by reflux extraction. Use 5 times the amount of 70% ethanol aqueous solution as the extractant, condense with running water, reflux extraction in a water bath at 90°C for 12 hours, and extract twice. After being adsorbed by the carrier, it is dried, and the obtained solid is the leaf alcohol extract of the flax plant.
[0035] The qualitative detection of total alkaloids in the extract was carried out according to Xie Zhongquan (2001) bismuth potassium iodide reagent method; the quantitative determination was carried out according to Miao Mingsan et al. The determination of flavonoids in the extract refers to the colorimetric method of Guo Yajian (2006). That is, aluminum nitrate was used for color development, rutin was used as the standard sample, and A510 was determin...
Embodiment 2
[0050] Preparation and application of embodiment 2 feed additive
[0051] 1, the preparation of flax leaf alcohol extract
[0052] Take 1000g fresh leaves of flax (oil-fiber dual-purpose flax), wrap them with filter paper, and extract them by reflux extraction. Use 10 times the amount of absolute ethanol as the extractant, condense with running water, reflux extraction in a water bath at 90°C for 1 hour, extract 3 times, and concentrate the extract to a paste by rotary evaporation, then put it in a vacuum drying oven to dry or carrier Drying after adsorption, the obtained solid is the flax leaf alcohol extract.
[0053] 2. Preparation of feed additives
[0054] Accurately weigh the above-mentioned flax (oil and fiber dual-purpose flax) leaf alcohol extract, lipoic acid, pantothenic acid, nicotinamide, biotin, vitamin E and wheat flour according to the following mass ratio 300:100:50:50:1.0:300:600 amount, uniformly mixed to obtain the feed additive.
[0055] 3. The effect ...
Embodiment 3
[0063] Preparation and application of embodiment 3 feed additives
[0064] 1, the preparation of flax leaf alcohol extract
[0065] Take 1000g fresh leaves of flax (linseed for oil), wrap them in filter paper, and extract them by reflux extraction. Use 7 times the amount of 95% ethanol as the extractant, condense with running water, reflux in a water bath at 90°C for 3 hours, extract 3 times, and concentrate the extract to a paste by rotary evaporation, then put it in a vacuum drying oven to dry or carrier Drying after adsorption, the obtained solid is the flax leaf alcohol extract.
[0066] 2. Preparation of feed additives
[0067] Accurately weigh flax (linseed for oil) leaf alcohol extract, lipoic acid, pantothenic acid, nicotinamide, biotin, vitamin E and wheat flour according to the following mass ratio 300:100:50:50:1.0:300:600, evenly The feed additive is obtained by mixing.
[0068] 3. Effects of feed additives on the content of DHA and EPA in quail eggs and the co...
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