A kind of preparation method and application of microbial fertilizer
A microbial fertilizer and seed technology, applied in botany equipment and methods, applications, organic fertilizers, etc., can solve the problems of high price of Streptomyces luteus bacteria fertilizer, decreased soil fertility, soil degradation, etc.
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Embodiment 1
[0033] Streptomyces flavus CICC M 23627 was inoculated into a solid slant medium, and the medium formula was as follows: 200 g of potatoes, 10 g of sucrose, 1.0 g of dipotassium hydrogen phosphate, 1.5 g of magnesium sulfate, 20 g of agar, 1000 ml of water, pH 7.0. The culture temperature was 28°C, and after culturing for 96 hours, the slanted culture was washed into the sterilized seed medium with sterile water, and stirred evenly. The cultivation temperature is 28°C, the cultivation time is 120h, and the seeds are dried and crushed; the crushed seeds are inserted into the culture material and stirred evenly. The preparation of 1.0kg of microbial fertilizer requires the following ingredients: 800g of arable soil, 100g of calcium carbonate, 50g of rice flour, Cottonseed cake 50g, the moisture is adjusted to 50%; the method of open culture in a shallow dish, the thickness of the culture material is 3cm, the culture temperature is 30 ° C, and the culture is cultured for 120h, the...
Embodiment 2
[0035] Streptomyces flavus CICC M 23627 was inoculated into a solid slant medium, and the medium formula was as follows: 200 g of potatoes, 15 g of sucrose, 0.5 g of dipotassium hydrogen phosphate, 1.0 g of magnesium sulfate, 20 g of agar, 1000 ml of water, pH 7.2. The culture temperature was 32°C, and after culturing for 96 hours, the slanted culture was washed into the sterilized seed medium with sterile water, and stirred evenly. The cultivation temperature is 32°C, the cultivation time is 96h, the seeds are dried and pulverized; the pulverized seeds are inserted into the culture material, and stirred evenly. The following ingredients are required to prepare 1.0kg of microbial fertilizer: 850g of arable soil, 50g of calcium carbonate, 50g of rice flour, Cottonseed cake 50g, moisture adjusted to 55%; the method of open culture in a shallow dish, the thickness of the culture material is 4cm, the culture temperature is 30°C, and the culture is 120h, the culture material is whit...
Embodiment 3
[0037] Streptomyces flavus CICC M 23627 was inoculated into solid slant medium, and the medium formula was as follows: potato 200g, sucrose 20g, dipotassium hydrogen phosphate 2.0g, magnesium sulfate 0.5g, agar 20g, water 1000ml, pH 7.0. The culture temperature was 30°C, and after culturing for 120 hours, the slanted culture was washed into the sterilized seed medium with sterile water, and stirred evenly. The culture temperature is 28°C, the culture time is 120h, and the seed medium is dried and crushed; the crushed seed culture medium is inserted into the culture material and stirred evenly. The following culture materials are required to prepare 1.0kg of microbial fertilizer: 750g of ploughing soil, 50g of calcium carbonate , 100 g of rice flour, 100 g of cottonseed cake, and the moisture content was adjusted to 60%; the culture was carried out in a shallow dish, the thickness of the culture material was 5 cm, the culture temperature was 30 °C, and the culture was cultured f...
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