Method for auxiliary identification of drought resistant maize

A technology for auxiliary identification and corn, applied in botany equipment and methods, horticulture, germination equipment, etc., can solve the problems of cumbersome and complicated measurement procedures, unsuitable for drought resistance identification, and little application value, so as to reduce work intensity and operation Less difficult, reliable results

Inactive Publication Date: 2013-02-20
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

Moreover, the determination procedure is cumbersome and time-consuming, and it is not suitable for a large number of drought resistance identification, so it is of little value in production practice

Method used

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  • Method for auxiliary identification of drought resistant maize
  • Method for auxiliary identification of drought resistant maize

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Embodiment 1

[0027] Embodiment 1, application method of the present invention identifies drought-resistant corn

[0028] 1. Identify each corn variety as follows:

[0029] 1. Select 30 seeds of the same size and no damage to carry out the following disinfection: first soak for 2 minutes with 70% (volume ratio) ethanol aqueous solution, then clean 3 times with distilled water, each 1 minute.

[0030] 2. Place the sterilized seeds in a 9cm-diameter petri dish, place double-layer filter paper in the petri dish as a germination bed, add 20mL 20% (20g / 100mL) PEG in the petri dish 6000 The water solution was used for drought stress as the experimental group; in addition, distilled water was used instead of PEG 6000 Parallel treatment of aqueous solution was used as a control group; both the experimental group and the control group germinated in a dark box at 25°C, the day of the drought treatment was regarded as the first day, and the number of germination was investigated every 2 days from the...

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Abstract

The invention discloses a method for auxiliary identification of drought resistant maize. The method includes the following process A consisting of: using filter paper as a germination bed, adding a PEG6000 aqueous solution with a concentration of 20g / 100mL, placing maize seeds to be tested on the filter paper to serve as a test group; replacing the PEG6000 aqueous solution with distilled water to serve as a control group; counting germination rates on the second day, the fourth day, the sixth day and the eighth day of the test, and calculating sprout index of drought resistance, with the sprout index of drought resistance being a ratio of a test group germination index to a control group germination index and a germination index expressed by the formula of: a germination index=(1.00)*nd2+(0.75)*nd4+(0.50)*nd6+(0.25)*nd8, and with the nd2, nd4, nd6, and nd8 being the germination rates on the second day, the fourth day, the sixth day and the eighth day of the test respectively; and taking the to-be-tested maize with a sprout index of drought resistance over 0.65 as the candidate germination stage drought resistant maize. With the method provided by the invention, drought resistance identification of maize varieties can be completed in a laboratory. And the method has the advantages of simplicity, practicability, less time and labor consumption, easy operation, good test repeatability, and reliable result, thereby reducing work intensity and improving work efficiency.

Description

technical field [0001] The invention relates to a method for assisting identification of drought-resistant corn. Background technique [0002] Corn is an important grain, feed and industrial raw material crop in my country, and occupies a pivotal position in China's agricultural production. Since 2004, the area of ​​corn planted in my country has been about 25 million hectares, and the annual output has accounted for 29.2% of the total output of grain crops. [0003] Drought is a global problem. The world's arid and semi-arid areas account for about one-third of the total land area. my country's arid and semi-arid land areas account for about 50% of the country's total area. Drought has become a limitation for my country and other countries in the world. An important factor for high and stable corn yields. Spring drought often occurs in northern China, which affects the germination of corn seeds, hinders the growth of seedlings, has few roots and short roots, weak seedlings...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01G1/00
Inventor 杨德光边鸣镝朴明鑫王永力李钊
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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