Method for cultivating regeneration plants of cotton
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A technology for regenerating plants and plants, applied in the biological field, can solve the problems of unsuccessful transplanting of resistant seedlings, underdeveloped root system, weak growth ability, etc., and achieve the effect of improving the survival rate of transplanting, simple operation and strong practicability
Active Publication Date: 2013-03-20
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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Problems solved by technology
However, transplanting by hardening seedlings directly after rooting will easily cause a large number of resistant seedlings to be transplanted unsuccessfully, because many regenerated plants become weak in growth ability and underdeveloped in the root system during the long-term tissue culture, resistance screening and differentiation process. , even vitrified
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Embodiment 1
[0033] Embodiment 1, Agrobacterium-mediated transplantation of regenerated cotton plants
[0034] 1. Agrobacterium-mediated genetic transformation of cotton to obtain regenerated cotton plants
[0035] Among the successful cases reported so far, the cotton recipient variety is mainly Kezi cotton, but also includes some other varieties, such as Zhongmian 12, Simian 3, Jinmian 7, Huakang 6, etc., cultivated in India. Cotton varieties Anjli (LRK516) and LRA 5166, etc. Genetic transformation mostly uses improved Agrobacterium tumefaciens (containing Ti plasmid), such as LBA4404, PGV2260, EMH105, etc., but different strains have different affinity to different cotton varieties, which directly affects the transformation efficiency. The explants used are very wide, including leaves, stem segments, hypocotyls, petioles, cotyledons, immature embryos, callus or mature seeds, but the most commonly used is still the hypocotyl. The media components, hormone types, culture conditions, etc...
Embodiment 2
[0077] Embodiment 2, microprojectile bombardment method mediates the transplanting of regenerated cotton plants
[0078] 1. Regenerated cotton plants mediated by microprojectile bombardment
[0079] The commonly used receptors for genetic transformation of cotton by particle gun bombardment include shoot apical meristem, embryogenic callus and suspension culture cells. The cotton recipient varieties reported to be successful include upland cotton and sea island cotton. Intrinsic factors of recipient cells and particle gun bombardment parameters will affect the transformation efficiency of exogenous genes. Factors of the recipient itself include the type of explant, the physiological state of the cell, the potential regeneration ability of the cell, the treatment of the cell before and after bombardment, and the acceptance of foreign DNA in the intracellular environment. The bombardment parameters of the gene gun generally take into account the bombardment pressure, bombardme...
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Abstract
The invention discloses a method for cultivating regeneration plants of cotton. The method for cultivating target cotton plants, provided by the invention, comprises the following steps of: (1) firstly, removing stem tips, branches and leaves as well as fibrous roots of a target cotton plant, and retaining 1-2 white fibrous roots or basal parts of the fibrous roots to obtain a main body; and secondly, cutting off along a first section, which is close to the root part, of the main body and retaining the first section, provided with the root, of the main body to obtain a plant to be subjected to subculture; (2) culturing the plant to be subjected to subculture in a regeneration plant subculture medium to obtain a regeneration plant subjected to subculture again; and (3) hardening and transplanting the regeneration plant subjected to the subculture again to obtain the transplanted cotton plant, wherein the target cotton plant is the regeneration cotton plant obtained after tissue culture. Proven by experiments, the cultivation method for cultivating the regeneration plants of cotton has the capability of greatly increasing the survival rate of transplanting of the regeneration plants of cotton. The method disclosed by the invention is simple to operate, strong in practicability and is conductive to improving the genetic transformation efficiency of the cotton.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating cotton regenerated plants. Background technique [0002] Cotton is an important raw material for the textile industry and fine chemicals, as well as an important strategic material. Genetically modified cotton was one of the first crops to be grown commercially. In 2011, the planting area of transgenic cotton in the world reached 24.7 million hectares, accounting for more than 80% of the total cotton area of 30 million hectares. [0003] The use of biotechnology to transfer genes for good traits into cotton can break species boundaries and expand genetic resources. [0004] At present, the commonly used genetic transformation methods for cultivating new transgenic cotton varieties through biotechnology include Agrobacterium-mediated method, microprojectile bombardment method and pollen tube passage method, etc. [0005] The Agrobacterium-mediated trans...
Claims
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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王志兴唐巧玲王旭静
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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