Coconut tree stem essential oil extract and application thereof in Oryctes rhinoceros(Linnaeus) attractant
A technology for extracting essential oils and rhinocarpus, which is applied in the field of extraction and application of plant-derived drugs, can solve the problems of unreasonable utilization, harm to the heart leaves and young tissues of coconuts, and less release of natural enemies, and achieve good results. Environmental and ecological benefits, good attracting effect, effect of simple preparation method
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Embodiment 1
[0018] Preparation of Coconut Stem Essential Oil Extract by Steam Distillation:
[0019] Take 5 kg of dead coconut stems, clean them indoors, chop them up, put them in a distillation bottle, and continue distillation with steam distillation for 24 hours. The extract was placed in a rotary evaporator and concentrated at 35°C until the extract became oily and no solvent was precipitated out to obtain the coconut stem essential oil extract.
[0020] Determination of field application effect of coconut stem essential oil extract:
[0021] Choose the dosage of 0.5ml / mu, 1 ml / mu and 1.5 ml / mu, hang the extract on the vertical trap of Rhinoceros bicariae, and set up a trap for each mu. In order to ensure the experimental effect, the experimental site is selected At the experimental base of the Coconut Research Institute of the Chinese Academy of Tropical Agricultural Sciences, each treatment was placed in a checkerboard manner, repeated 3 times, and the number of adults of Rhinocero...
Embodiment 2
[0023] Preparation of Coconut Stem Essential Oil Extract by Soxhlet Extraction:
[0024] Take 5 kg of dead coconut stems, wash them indoors, dry them at 40°C and crush them, put them in a Soxhlet extractor, and use petroleum ether for Soxhlet extraction for 18 hours, collect the extracts and place them in a rotary evaporator, and depressurize at 45°C When it is concentrated until the extract becomes oily and no solvent is separated out, the essential oil extract of the coconut stem is obtained.
[0025] Determination of field application effect of coconut stem essential oil extract:
[0026] Test method and test site are the same as implementation example 1, and the results are as follows figure 2 shown.
Embodiment 3
[0028] Preparation of coconut stem essential oil extract by solvent maceration:
[0029] Collect the dead coconut stem tissue, wash it with clean water, put it in the shade of the room, put it into a blast drying box for drying at 45°C, cool and crush it to obtain the coconut stem powder; weigh 1kg of the coconut stem powder and place it in soaking In the cylinder, add the organic solvent diethyl ether according to the volume ratio of 1:10, soak for more than 24 h, and obtain the coconut stem ether extract after filtering; transfer the coconut stem extract to a rotary evaporator, and concentrate under reduced pressure at 35°C When the extract turns into a viscous paste and no solvent is separated out, the coconut stem essential oil extract is obtained.
[0030] Determination of field application effect of coconut stem essential oil extract:
[0031] Test method and test site are the same as implementation example 1, and the results are as follows image 3 shown.
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