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Chroococcus culture medium formula

A culture medium, the technology of Chromococcus, applied in the field of medium formula of Chromococcus, to achieve the effects of fast growth rate, high survival rate and less pollution

Active Publication Date: 2014-08-13
LIYANG TECH DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports about the culture method of Chromococcus at present, and there are even fewer reports on the culture medium specially used for Chromococcus

Method used

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  • Chroococcus culture medium formula

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Prepare the culture medium with the following formula:

[0011] NaCl 1000mg / L, NH 4 NO 3 100mg / L, K 2 SO 4 800mg / L, CaCl 2 .2H 2 O 300mg / L, MgSO 4 .7H 2 O 200mg / L, K 2 HPO 4 600mg / L, KI 0.5mg / L, H 3 BO 3 10mg / L, MnSO 4 .4H 2 O 30mg / L, ZnSO 4 .7H 2 O 0.4mg / L, CuSO 4 .5H 2 O 0.05mg / L, CoCl 2 .6H 2 O 0.05mg / L, Mo(NO 3 ) 3 .5H 2 O 4mg / L;

[0012] Niacin 0.2mg / L, vitamin B 6 0.1mg / L, vitamin B 1 0.1mg / L, NNA 0.05mg / L, IBA 0.02mg / L.

[0013] Chromococcus micrococcus in the logarithmic growth phase was inoculated into the medium for cultivation, and the light was 10 hours a day, and the temperature of the incubator was controlled at about 35 degrees Celsius, and the cell density of Chromococcus micrococcus in the medium was recorded every 2 days.

Embodiment 2

[0015] NaCl 1200mg / L, NH 4 NO 3 105mg / L, K 2 SO 4 1000mg / L, CaCl 2 .2H 2 O 400mg / L, MgSO 4 .7H 2 O 300mg / L, K 2 HPO 4 700mg / L, KI 0.55mg / L, H 3 BO 3 12mg / L, MnSO 4 .4H 2 O 35mg / L, ZnSO 4 .7H 2 O 0.5mg / L, CuSO 4 .5H 2 O 0.1mg / L, CoCl 2 .6H 2 O 0.1mg / L, Mo(NO 3 ) 3 .5H 2 O 5mg / L;

[0016] Niacin 0.3mg / L, vitamin B 6 0.2mg / L, vitamin B 1 0.2mg / L, NNA 0.08mg / L, IBA 0.04mg / L.

[0017] Chromococcus micrococcus in the logarithmic growth phase was inoculated into the medium for cultivation, and the light was 10 hours a day, and the temperature of the incubator was controlled at about 35 degrees Celsius, and the cell density of Chromococcus micrococcus in the medium was recorded every 2 days.

Embodiment 3

[0019] NaCl 1100mg / L, NH 4 NO 3 102mg / L, K 2 SO 4 900mg / L, CaCl 2 .2H 2 O 350mg / L, MgSO 4 .7H 2 O 250mg / L, K 2 HPO 4 650mg / L, KI 0.52mg / L, H 3 BO 3 11mg / L, MnSO 4 .4H 2 O 33mg / L, ZnSO 4 .7H 2 O 0.45mg / L, CuSO 4 .5H 2 O 0.075mg / L, CoCl 2 .6H 2 O 0.075mg / L, Mo(NO 3 ) 3 .5H 2 O 4.5mg / L;

[0020] Niacin 0.25mg / L, Vitamin B 6 0.15mg / L, vitamin B 1 0.15mg / L, NNA 0.06mg / L, IBA 0.03mg / L.

[0021] Chromococcus micrococcus in the logarithmic growth phase was inoculated into the medium for cultivation, and the light was 10 hours a day, and the temperature of the incubator was controlled at about 35 degrees Celsius, and the cell density of Chromococcus micrococcus in the medium was recorded every 2 days.

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Abstract

The invention provides a Chroococcus culture medium. The formula of the Chroococcus culture medium comprises 1000-1200mg / L of NaCl, 100-105mg / L of NH4NO3, 800-1000mg / L of K2SO4, 300-400mg / L of CaCl2.2H2O, 200-300mg / L of MgSO4, 600-700mg / L of K2HPO4, 0.5-0.55mg / L of KI, 10-12mg / L of H3BO3, 30-35mg / L of MnSO4.4H2O, 0.4-0.5mg / L of ZnSO4.7H2O, 0.05-0.1mg / L of CuSO4.5H2O, 0.05-0.1mg / L of CoCl2.6H2O, 0.2-0.3mg / L of nicotinic acid, 0.1-0.2mg / L of vitamin B6, 0.1-0.2mg / L of vitamin B1, 0.05-0.08mg / L of NNA and 0.02-0.04mg / L of IBA. The Chroococcus culture medium has the characteristics of high survival rate and fast growth rate when Chroococcus is cultured by the medium, and cannot be polluted by other bacteria.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a culture medium formula of Chromococcus. Background technique [0002] Chroococcus minutus belongs to Cyanophyta, Chroococcus class, Chroococcus order, Chroococcus family, and Chroococcus genus. The cells are spherical, single or not separated into two connected hemispheres after division, and the cell diameter is 2.5-4.0 μm. The alginate was extremely thin, colorless and transparent; the protoplasts were uniform and blue-green. The content of crude protein contained in Chromococcus micrococcus is very high, reaching 61.82% of the dry matter, and the total fat content is very low, accounting for only about 1% of the dry matter. In addition, Chromococcus micrococcus contains a variety of amino acids, among which the content of glutamic acid is the highest, followed by aspartic acid, alanine and leucine; the content of cystine, histidine and methionine lower. Chromococcus micrococcus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 彭江晨
Owner LIYANG TECH DEV CENT
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