Interest protein preparation method and purpose thereof

Abstract

The invention relates to an interest protein preparation method and a purpose thereof. The preparation method includes the steps of obtaining interest protein nucleotide sequence fragments, constructing interest protein recombinant expression vectors, expressing interest protein in an inducible mode, purifying the interest protein and cutting purified products. The fronts of the interest protein nucleotide sequence fragments are inserted into monomer cutting recognition sites. The interest protein is obtained through polymerase chain reaction (PCR) amplification, and is introduced into monomer cutting recognition sites through primers. The interest protein can be human nerve growth factors such as thymosin, tiger stripe pancreatic polypeptide and tiger stripe analgesic peptide. By adopting the interest protein preparation method, obtained interest protein is high in expression quality, easy to purify and capable of reducing the difficulty for folding of interest protein disulfide bond renaturation and conformation. Genetic expression products are interest protein monomer, extra amino acid residue is carried, and thus not only is the good biology activity of interest protein products ensured, but also multicopy cutting cost is saved, the purification is simple and the mass production is facilitated.

Description

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AI Technical Summary

Benefits of technology

This patented technology helps create stable proteins with reduced reductive steps that can be easily separated from other parts during production process or use for research purposes. By reducing this problem it makes producing these products easier and more efficient than traditional methods like lyophilization.

Problems solved by technology

This patents describes various technical problem addressed during this patented research: finding ways to improve or preserve neurotrophic factors like brain derived fibroblast cell lineage proteins while reducing their production costs compared to traditional techniques.

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