Molecular marker for co-separating soybean anti-phytophthora megasperma candidate gene RpsWD15-1 and application of molecular marker for co-separating soybean anti-phytophthora megasperma candidate gene RpsWD15-1
A technology of anti-phytophthora root rot and molecular markers, which is applied in the field of agricultural biotechnology engineering and genetic breeding of crop resistance, can solve the problems of ineffective resistance to soybean Phytophthora populations, improve breeding efficiency, reduce yield loss, and accelerate The effect of the work process
Inactive Publication Date: 2013-03-27
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology
A large number of studies have shown that among the 15 disease resistance genes identified abroad (except the Rps gene in cv.Waseshiroge), except for Rps1c and Rps1k, none of them can effectively resist the population of Phytophthora sojae in diseased areas in my country
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Embodiment 1
[0046] Example 1 Acquisition of a new candidate gene RpsWD15-1 for soybean resistance to Phytophthora root rot and its co-segregated molecular marker Sattwd15-28
[0047] 1.1 Genetic analysis of resistance in Wandou 15
[0048] Using the disease-resistant variety Wandou 15 as the male parent and the susceptible variety Williams (rps) as the female parent, a hybrid combination was prepared to produce F 1 Generation, F 1 F 2 Generation, F 2 102 F 2:3 The family is used as a population for resistance genetic analysis, disease resistance gene identification and mapping. Using hypocotyl wound inoculation, 20-30 plants in each family were identified for genetic analysis of resistance to strain PsMC1. After inoculation, they were kept moist for 48 hours at 25°C and the humidity was 80-100%, and then transferred to the greenhouse for cultivation. Disease investigation was carried out 6 days later. Referring to the evaluation criteria of Gordon (2006) and other methods, that is, ...
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The invention relates to a molecular marker for co-separating a soybean anti-phytophthora megasperma candidate gene RpsWD15-1 and an application of the molecular marker for co-separating the soybean anti-phytophthora megasperma candidate gene RpsWD15-1. A disease-resistant variety wan bean 15 is taken as a male parent, a susceptible variety Williams (rps) is taken as a female parent, a generation F1 is generated through hybridizing, a population F2:3 is generated through self-crossing to be used as a mapping population, and an acknowledged soybean simple sequence repeats (SSR) marker and the SSR marker which is newly developed according to a soybean genomic sequence are utilized to carry out genetic mapping and fine mapping on an anti-disease gene which contains the wan bean 15. The anti-disease gene RpsWD15-1 is positioned on a chromosome No.17 and is positioned between the makers of Sattwd15-24 / 25(0.5centimeter) and Sattwd15-47(0.8centimeter). Moreover, a molecular marker Sattwd15-28 for co-separating the gene RpsWD15-1is also obtained. The marker can be used for soybean anti-phytophthora megasperma marker-assisted selection.
Description
technical field [0001] The invention belongs to the fields of agricultural biotechnology engineering and crop disease-resistant genetic breeding, and specifically relates to a molecular marker co-separated with soybean Phytophthora root rot-resistant candidate gene RpsWD15-1 and its application. Background technique [0002] Phytophthora sojae (Phytophthora sojae Kaμfmann & Gerdemann) caused by Phytophthora sojae Kaμfmann & Gerdemann is one of the main diseases that endanger soybean production. In recent years, the disease has become more and more harmful in Heilongjiang Province, the main soybean producing area, and the area of soybean damage has reached 15×10 4 hectare. The disease can cause harm at any growth stage of soybeans. Generally, the yield of the field is reduced by 10-30%, and the serious plot can reach 60-90%, or even cause the production to be terminated, which poses a serious threat to soybean production. At present, soybean Phytophthora root rot has beco...
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IPC IPC(8): C12N15/11C12Q1/68A01H1/04
Inventor 朱振东张吉清王晓鸣夏长剑段灿星
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI