Method for preparing high-purity astaxanthin extract

An astaxanthin and extract technology, which is applied in the field of preparing high-purity astaxanthin extract, can solve problems such as inability to realize industrialized production, and achieve the effects of good promotion and application prospect, good health care function, and high development value.

Inactive Publication Date: 2013-04-03
NINGBO HONGLONG BIO TECH
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  • Abstract
  • Description
  • Claims
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Although large-scale preparative chromatography can achieve the separation of

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  • Method for preparing high-purity astaxanthin extract
  • Method for preparing high-purity astaxanthin extract

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Example Embodiment

[0036] Example 1. A method for preparing a high-purity astaxanthin extract, followed by the following steps:

[0037] 1) Take 1mL of natural astaxanthin oil produced by Ningbo Honglong Biotechnology Co., Ltd. and dilute it with 5mL of absolute ethanol to obtain the diluted astaxanthin crude extract;

[0038] Take 1mL of the diluted crude astaxanthin extract and add 4mL12mmol / L NaOH-anhydrous ethanol solution, mix well and place in a dark place at room temperature (25℃) to saponify for 1h to obtain a saponification solution;

[0039] 2). Add the saponification liquid obtained in step 1) to a dichloromethane-water solution (obtained by mixing 5 mL of dichloromethane + 5 mL of water), mix well, stand still for layering, and discard the upper liquid. The lower liquid was concentrated at 45°C under vacuum (0.09MPa) to obtain an extract (about 200mg);

[0040] 3). Dissolve the extract obtained in step 2) in 1 mL of n-hexane: acetone=4:1 (volume ratio) solution, and load all samples onto a 2...

Example Embodiment

[0062] Example 2. A method for preparing a high-purity astaxanthin extract, followed by the following steps:

[0063] 1) Take 0.1 mL of the natural astaxanthin oil produced by Ningbo Honglong Biotechnology Co., Ltd. and dilute it with 0.5 mL of absolute ethanol to obtain the diluted astaxanthin crude extract;

[0064] Take 0.1mL of diluted astaxanthin crude extract and add 0.4mL6mmol / L NaOH-anhydrous ethanol solution, mix well and place in a dark place at room temperature (30℃) for 0.5h saponification to obtain saponification liquid;

[0065] 2). Add the saponification liquid obtained in step 1) to a dichloromethane-water (2mL+1mL) solution, mix well and then stand still for layering, and discard the upper liquid. The lower liquid was concentrated under vacuum at 40°C to obtain the extract;

[0066] 3). Dissolve the extract obtained in step 2) in 1 mL of n-hexane:acetone=4:1 (volume ratio) solution, and load all the samples onto a 2.5cm×40cm silica gel column. The silica gel column u...

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Abstract

The invention discloses a method for preparing a high-purity astaxanthin extract. The method comprises the following steps in sequence: (1) taking a crude extract of astaxanthin, diluting with absolute ethyl alcohol, adding a NaOH- absolute ethyl alcohol solution with concentration of 6-12 mmol/L, and then placing at a dark place for saponification at room temperature; (2) adding the obtained saponification solution into a methylene dichloride-water solution, standing for layering, and concentrating the lower layer liquid to obtain an extractum; (3) dissolving the obtained extractum into a solution with the volume ratio of normal hexane to acetone being 4 to 1, and applying onto a silicagel column; sequentially performing the following elution: taking the solution with the volume ratio of normal hexane to acetone being 4 to 1 as the elution solution I for elution to obtain a yellow fraction, taking the solution with the volume ratio of normal hexane to acetone being 1 to 1 as the elution solution II for elution to obtain an orange fraction, and eluting with methyl alcohol till the silicagel column is colorless; and (4) performing vacuum concentration on the orange fraction, thereby obtaining an aubergine extractum which is the high-purity astaxanthin extract.

Description

technical field [0001] The invention belongs to the separation and purification of natural products, and mainly relates to a method for preparing high-purity astaxanthin extract. Background technique [0002] Astaxanthin is a red carotenoid with the chemical name 3,3’-dihydroxy-β,β’-carotene-4,4’-dione and the molecular formula C 40 h 52 o 4 (Formula 1). Astaxanthin has a wide range of uses in industry: first, astaxanthin has health functions such as anti-oxidation, lowering blood fat, anti-tumor, and anti-aging, and can be used in food, health care products, pharmaceuticals and cosmetics industries; second, astaxanthin Astaxanthin is a good food coloring agent, which can be used as a food coloring agent; thirdly, astaxanthin can also be used as a feed additive in the cultivation of ornamental fish and shrimp to make its color more vivid. In terms of sources, astaxanthin can be either artificially synthesized or extracted from living organisms. The application of artifi...

Claims

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Application Information

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IPC IPC(8): C07C403/24C09B61/00
Inventor 黄胜奎马建陈黎明
Owner NINGBO HONGLONG BIO TECH
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