Method for preparing broad-spectrum growth-promoting biocontrol bacillus B96-II fermentation broth

A technology of B96-II and Bacillus, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemicals for biological control, etc., can solve the problems of poor pertinence and broad spectrum, reduce production costs, and broaden good spectral effect

Inactive Publication Date: 2013-04-03
山西省农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of strong pertinence and poor broad-spectrum of the control objects of existing microorganisms, the present invention provides a method for preparing a broad-spectrum growth-promoting and anti-bacterial bacillus B96-II fermented liquid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation method of broad-spectrum growth-promoting and anti-bacillus B96-II fermented liquid comprises the following steps:

[0017] (1) Strain activation: insert the 3rd generation Bacillus B96-II into the pre-made PDA slant medium maintained at 10°C for 3 days, and culture it in a constant temperature incubator at 28°C for 3 days to obtain PDA slant bacteria kind;

[0018] (2) Production of B96-II seed liquid medium: take 200g of washed and peeled potatoes, cut into 1cm cubes, boil in 1L of water and then cook on low heat for 30 minutes, filter and dilute the filtrate to 1000mL with distilled water, add dihydrogen phosphate Mix 5g of potassium, 10g of sodium chloride, and 15g of glucose, and then sterilize at 121°C for 30 minutes to obtain the B96-II seed liquid culture medium;

[0019] (3) Making B96-II seed liquid: After the B96-II seed liquid culture medium is naturally cooled, insert the PDA slant strain prepared in step 1, and culture it on a shaker at 30...

Embodiment 2

[0023] The preparation method of broad-spectrum growth-promoting and anti-bacillus B96-II fermented liquid comprises the following steps:

[0024] (1) Strain activation: insert the second-generation Bacillus B96-II strain into the PDA slant medium prepared in advance for 4 days and keep the temperature at 7°C, and cultivate it in a constant temperature incubator at 28°C for 2 days to obtain PDA slant bacteria kind;

[0025] (2) Production of B96-II seed liquid medium: take 200g of washed and peeled potatoes, cut into 1cm cubes, boil in 1L of water and then cook on low heat for 30 minutes, filter and dilute the filtrate to 1000mL with distilled water, add dihydrogen phosphate Mix 10 g of potassium, 1 g of sodium chloride, and 20 g of glucose, and then sterilize at 121°C for 25 minutes to obtain B96-II seed liquid culture medium;

[0026] (3) Making B96-II seed liquid: After the B96-II seed liquid culture medium is naturally cooled, insert the PDA slant strain prepared in step ...

Embodiment 3

[0030] The preparation method of broad-spectrum growth-promoting and anti-bacillus B96-II fermented liquid comprises the following steps:

[0031] (1) Strain activation: insert the 3rd generation Bacillus B96-II strain into the PDA slant medium prepared in advance for 5 days and keep the temperature at 4°C, and cultivate it in a constant temperature incubator at 28°C for 2.5 days to obtain PDA slant bacteria kind;

[0032] (2) Production of B96-II seed liquid medium: take 200g of washed and peeled potatoes, cut into 1cm cubes, boil in 1L of water and then cook on low heat for 30 minutes, filter and dilute the filtrate to 1000mL with distilled water, add dihydrogen phosphate Mix 1g of potassium, 6g of sodium chloride, and 10g of glucose, and then sterilize at 121°C for 20 minutes to obtain B96-II seed liquid culture medium;

[0033] (3) Making B96-II seed liquid: After the B96-II seed liquid culture medium is naturally cooled, insert the PDA slant strain prepared in step 1, an...

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PUM

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Abstract

The invention relates to a method for preparing biocontrol strain fermentation broth and particularly relates to a method for preparing broad-spectrum growth-promoting biocontrol bacillus B96-II fermentation broth which can prevent and treat blight-based soil-borne diseases of vegetables and melons and has a growth promoting effect on a variety of vegetables. The method comprises the following steps of: activating a strain; preparing a B96-II strain liquid culture medium; preparing B96-II strain liquid; preparing a B96-II fermentation broth culture medium; and preparing the B96-II fermentation broth. The bacillus B96-II fermentation broth prepared by the method has good broad-spectrum performance, can prevent and treat the diseases of a variety of vegetables and melons, and has a growth promoting effect on a variety of vegetables, so that the bacillus B96-II fermentation broth is widely applicable to the prevention and treatment of the soil-borne diseases of the vegetables and melons.

Description

technical field [0001] The invention relates to a preparation method of a fermentation liquid of bio-control bacteria, specifically a broad-spectrum growth-promoting and anti-bacteria bacillus capable of preventing and controlling soil-borne diseases of vegetable melons, mainly Fusarium wilt, and having a growth-promoting effect on various vegetables. The preparation method of B96-II fermented liquid. Background technique [0002] Vegetables and fruits are an indispensable food for people's daily life. Vegetables and fruits are almost always planted wherever there is land. In recent years, with the continuous progress of facility agriculture, in order to facilitate production, marketing and transportation, the production mode of large-scale large-scale monoculture is increasing day by day, which provides a suitable host and environment for the propagation and spread of pathogenic bacteria, leading to the increasing severity of soil-borne diseases. Soil treatment with chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00A01P21/00C12R1/07
Inventor 马利平郝变青乔雄梧
Owner 山西省农业科学院
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