Waxy bacillus strain and microbial bactericide producing process with the strain

A Bacillus cereus, production method technology, applied in the fields of botanical equipment and methods, biocides, animal repellents, etc., can solve the problems of single disease type, poor broad spectrum, single variety, etc.

Inactive Publication Date: 2005-10-05
山西农业大学植物保护学院(山西省农业科学院植物保护研究所)
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each strain of the existing Bacillus cereus mainly has a control effect on a kind of disease of plants, and two kinds of diseases individually, and the growth-promoting effect is also that each strain works on individual 1-2 kinds of plants, so it can prevent and treat The type of disease is single, the growth-promoting effect is aimed at a single variety of plants or crops, and the broad-spectrum is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The production method for preparing microbial fungicides with B.C.98-I strains, the production process includes the preparation of slant strains, the preparation of shaker strains, the preparation of first-class strains and large tank fermentation, wherein the process conditions for the preparation of slant strains It is: PDA culture medium, 28 ℃, 48 hours; the technical conditions for the preparation of shaker strains are: No. ① medium, 28 ℃, shaker speed 150 rpm, 48 hours, No. ① medium: potato 20% , glucose 1.5%, sodium chloride 0.2%, potassium dihydrogen phosphate 0.1%, and the rest is water; the process conditions for the preparation of the first-grade strains are: medium No. ②, 28°C, fermenter speed 150 rpm, 48 hours . The pH value is 4, the ventilation rate is 1:1, and the culture medium of ② is: 0.2% corn flour, 0.2% soybean flour, 3% glucose, 0.1% sodium chloride, 0.1% potassium dihydrogen phosphate, 0.1% ammonium sulfate, and 0.1 peptone %, magnesium sulfate 0...

Embodiment 2

[0048] The production method for preparing microbial fungicides with B.C.98-I strains, the production process includes the preparation of slant strains, the preparation of shaker strains, the preparation of first-class strains and large tank fermentation, wherein the process conditions for the preparation of slant strains It is: PDA culture medium, 28 ℃, 48 hours; the technical conditions for the preparation of shaker strains are: No. ① medium, 42 ℃, shaker speed 200 rpm, 48 hours, No. ① medium: potato 20% , glucose 3.0%, sodium chloride 0.5%, potassium dihydrogen phosphate 0.6%, and the rest is water; the process conditions for the preparation of the primary strain are: medium No. ②, 42°C, fermenter speed 280 rpm, 48 hours , PH value 10, ventilation rate 1:1, ② medium: 0.5% corn flour, 0.5% soybean flour, 6% glucose, 0.8% sodium chloride, 0.8% potassium dihydrogen phosphate, 0.8% ammonium sulfate, peptone 0.8%, magnesium sulfate 0.05%, zinc sulfate 0.05%, all the other are wa...

Embodiment 3

[0050] The production method for preparing microbial fungicides with B.C.98-I strains, the production process includes the preparation of slant strains, the preparation of shaker strains, the preparation of first-class strains and large tank fermentation, wherein the process conditions for the preparation of slant strains It is: PDA culture medium, 28 ℃, 48 hours; the technical conditions for the preparation of shaker strains are: No. ① medium, 35 ℃, shaker speed 180 rpm, 48 hours, No. , glucose 2.0%, sodium chloride 0.3%, potassium dihydrogen phosphate 0.4%, and the rest is water; the process conditions for the preparation of the primary strain are: medium No. ②, 35°C, fermenter speed 220 rpm, 48 hours , PH value 7, ventilation volume 1:1, medium ②: 0.4% corn flour, 0.3% soybean flour, 4% glucose, 0.5% sodium chloride, 0.5% potassium dihydrogen phosphate, 0.5% ammonium sulfate, peptone 0.5%, magnesium sulfate 0.03%, zinc sulfate 0.03%, and the rest is water. The technologica...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a Bacillus cereus strain and microbial bactericide producing process with the strain. The Bacillus cereus strain is named Bacillus cereus 98-I and has good broad-spectrum property, high bacteriostatic activity, plant growth and germination promoting effect and plant resistance inducing effect. The microbial bactericide producing process with the strain includes slant culture of strain, shaking table preparation of strain, the first stage strain preparation and cylinder fermentation with the relevant parameters for every steps being described. The microbial bactericide has wide application in preventing and controlling soil-borne diseases of vegetable, melon, flower and lawn.

Description

technical field [0001] The invention relates to a bacterial strain, specifically a bacterial strain of Bacillus cereus, and also relates to a production method for preparing a microbial bactericide with the bacterial strain. Background technique [0002] Bacillus cereus belongs to the genus Bacillus. At present, there are domestic and foreign literature reports that different strains of Bacillus cereus are used for plant pathogens, disease control and growth promotion. Domestically, such as B.C.sp inhibits rice febrile bacteria, and B.C.S14 controls and promotes cotton diseases. B.C.R2 can prevent and control rice sheath blight, B.C.752 can promote the growth of rice, watermelon and corn, and can detoxify the spores of pathogens. B.C.CS51 can antagonize Narcissus brown spot. growth, B.C has antagonistic effects on wheat take-all and cotton wilt; foreign countries such as Bacillus cereus B.C.uw85, B.C.S18, B.C.W35, B.C.28, B.C.B 0 , B.C.DGA34, B.C.MS1-9, B.C.AS4-12 and othe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/00C12N1/20
Inventor 乔雄梧马利平高芬郝变青
Owner 山西农业大学植物保护学院(山西省农业科学院植物保护研究所)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap