Bactrian camel lysozyme protein gene, recombinant protein and cloning method thereof

Abstract

The invention relates to the technical field of genetic engineering and discloses a bactrian camel lysozyme protein gene, a recombinant protein and a cloning method thereof. The gene is a closely related gene which has the functions of resisting bacteria, diminishing inflammation and resisting viruses and can enhance the antibody activity and has a nucleotide sequence from 27-481st locus of the nucleotide in SEQ ID NO.1. The bactrian camel lysozyme sequence and an evolutionary tree thereof can intuitively show the difference between the bactrian camel lysozyme sequence and the protein sequence of other species, experimental data are provided for research on the hydrolysis of lysozyme of the bactrian camel and other species on the bacterial cell wall, a certain theoretical basis is provided for binding the lysozyme of the bactrian camel, other species and humans with viral protein with negative charges and inactivating the bound protein, and an important function of researching the functions of the lysozyme in human bacteria resistance, inflammation elimination and virus resistance; and therefore, the bactrian camel lysozyme protein gene has high application value.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, and relates to a Bactrian camel lysozyme protein gene, a recombinant protein and a cloning method thereof. Background technique [0002] Lysozyme (lysozyme), also known as muramidase or N-acetylmuramide glycanohydrlase, is a hydrolase that specifically acts on bacterial cell walls. Mainly by destroying the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall, the insoluble mucopolysaccharides in the cell wall are decomposed into soluble glycopeptides, resulting in the escape of the contents of the cell wall Bacteria dissolve. Lysozyme can also directly combine with negatively charged viral proteins, and form double salts with DNA, RNA, and apoproteins to inactivate the virus. Therefore, the enzyme has antibacterial, anti-inflammatory, antiviral and other effects. [0003] People mainly drink camel milk in the form of fresh milk, and have found...

AI Technical Summary

Problems solved by technology

[0007] The content of lysozyme in Bactrian camel milk is significantly higher than that of cow milk and buffalo milk, and it can effectively inhibit the growth of pathogenic bacteria, but there are few studies on lysozyme in camel milk at home and abroad.

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