Novel technology method for extracting baicalin from wild scutellaria baicalensis

A technology of baicalin and a new method, which is applied in the field of high-purity baicalin, can solve the problems of low yield and long extraction time, and achieve the effect of simple and easy operation, no chemical residue, and low cost

Active Publication Date: 2013-04-17
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In China, it is generally extracted with water, adjusted to acid to precipitate, separated from the precipitate, washed and dried to produce baicalin, which has the disadvantages of long extraction time and low yield.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: A kind of new method that adopts biological enzymolysis, ultrasonic extraction, polyamide-macroporous resin combined technology to obtain high-purity baicalein, its steps are as follows:

[0023] (1) 50g of Scutellaria baicalensis is crushed to 10 meshes, using compound cellulase, the enzyme dosage is 1% of the substrate, the enzymolysis temperature is 40°C, and the precipitation is kept for 2h;

[0024] (2) adding 70% ethanol, ultrasonic extraction, ultrasonic frequency 50KHZ, solid-liquid ratio 1:8, ultrasonic time 50min, thin-layer chromatography detection and analysis, recovery of ethanol, to obtain a crude extract;

[0025] (3) Leave the extract at about 4°C for 4 hours, and filter to obtain the supernatant;

[0026] (4) Preliminary refining of polyamide:

[0027] Mix the obtained solution into dry 60-mesh polyamide powder, wash with water to remove water-soluble impurities, then elute with 20%, 40%, 60%, and 80% ethanol in sequence, collect the elue...

Embodiment example 2

[0034] Implementation Case 2: A new method for obtaining high-purity baicalin by using biological enzymatic hydrolysis, ultrasonic extraction, and polyamide-macroporous resin combined technology. The steps are as follows:

[0035] (1) 100g of Scutellaria baicalensis is crushed to 10 mesh, the enzyme dosage is 0.5% of the substrate, the enzymatic hydrolysis temperature is 45°C, and the precipitation is kept for 4 hours:

[0036] (2) adding 75% ethanol, ultrasonic extraction, ultrasonic frequency 60KHZ, solid-liquid ratio 1:9, ultrasonic time 60min, thin-layer chromatography detection and analysis, recovery of ethanol, to obtain a crude extract;

[0037] (3) Leave the extract at about 4°C for 5 hours, and filter to obtain the supernatant;

[0038] (4) Preliminary refining of polyamide:

[0039] Mix the obtained solution into dry 80-mesh polyamide powder, wash with water to remove water-soluble impurities, then elute with 20%, 40%, 60%, and 80% ethanol in sequence, collect the e...

Embodiment example 3

[0047] A new method for obtaining high-purity baicalin by using biological enzymatic hydrolysis, ultrasonic extraction, and polyamide-macroporous resin combined technology, the steps are as follows:

[0048] (1) 100g of Scutellaria baicalensis is crushed to 20 meshes, using compound cellulase, the enzyme dosage is 0.8% of the substrate, the enzymatic hydrolysis temperature is 50°C, and the precipitation is incubated for 3 hours;

[0049] (2) adding 80% ethanol, ultrasonic extraction, ultrasonic frequency 80KHZ, solid-liquid ratio 1: 10, ultrasonic time 80min, thin-layer chromatography detection and analysis, recovery of ethanol, to obtain a crude extract;

[0050] (3) Leave the extract at about 4°C for 6 hours, and filter to obtain the supernatant;

[0051] (4) Preliminary refining of polyamide:

[0052] Mix the obtained solution into dry 60-mesh polyamide powder, wash with water to remove water-soluble impurities, then elute with 20%, 40%, 60%, and 80% ethanol in sequence, c...

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting baicalin from wild scutellaria baicalensis in great khingan, in particular to a novel method for obtaining high-purity baicalin through biological enzymolysis, ultrasonic extraction and a polyamide-macroporous resin coupling technique. The method has the advantages that extraction is quick and convenient; a purification effect is obvious; and the method can be applicable to industrial production. 1, a biological enzymolysis technique is adopted to ensure that effective components in cells are dissolved out fully; 2, an ultrasonic extraction technique is adopted, so that the heating time of the effective components is shortened; the activity loss is less; and the bioactivity of baicalin is high; and 3, the polyamide-macroporous resin coupling technique is combined for use, so that the effective components are further purified; resin can be reused; the production cost is lowered greatly; and the method is applicable to industrial use. The method adopts the biological enzymolysis technique, so that the effective components are dissolved out as much as possible; then the ultrasonic extraction is conducted; finally a polyamide-macroporous resin secondary purification technique is adopted, and recrystallization is conducted; and the purity of baicalin is increased to 98.6%.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting baicalin from wild baicalin in Daxing'an Mountains, in particular to a method for obtaining high-purity baicalin by using bioenzymolysis, ultrasonic extraction, and polyamide-macroporous resin combination technology new method. Background technique: [0002] Scutellaria baicalensis is a tall herb with a stem height of 60-150 cm and villous hairs. Pinnately compound leaves. Leaflets 21-31, ovate-lanceolate or elliptic, 7-30 mm long, 4-10 mm wide, with white villous hairs on both sides. Leaf rachis has villous hairs. Stipules narrowly lanceolate, about 6 mm long, with white villous hairs. Racemes axillary. There are strip bracts under the flowers. Calyx tubular, about 5mm long, teeth short, white villous. Corolla yellowish white, flag petals without claws, longer than wing petals and keel petals, wing petals and keel petals with long claws. Ov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 张亚红姚德坤姚德利万莉焦龙
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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