Method of using glycine modified quantum dot probes to mark living cell

A technology of quantum dots and glycine, which is applied in the field of glycine-modified quantum dot probes for labeling living cells, can solve problems such as the need for improvement of quantum dot probe modification methods, and achieves short reaction time, low requirements for experimental conditions, and short incubation time. Effect

Inactive Publication Date: 2013-04-17
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Therefore, the modification method of q...

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  • Method of using glycine modified quantum dot probes to mark living cell
  • Method of using glycine modified quantum dot probes to mark living cell

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[0024] The quantum dot-glycine probe can be applied to the labeling of various living cells, and the usage method is similar. However, depending on the cell type and culture method (suspension, adherence), the labeling effect may be different, and the required probe concentration should also be adjusted as needed. And the quantum dot-glycine probe obtained by coupling the carboxyl group of the water-soluble quantum dot with the carboxyl functional group and the amino group of glycine, and the quantum dot-glycine probe obtained by coupling the amino group of the water-soluble quantum dot with the amino functional group and the carboxyl group of glycine obtained in different cells The results achieved may vary between experiments and experimental conditions. The following takes the primary cultured myocardium as an example for specific description.

[0025] (1) Reagents and instruments:

[0026] 1. Cells: primary cultured SD rat suckling rat cardiomyocytes

[0027] 2. Main re...

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Abstract

The invention discloses a method of using glycine modified quantum dot probes to mark a living cell, which comprises the following steps: coupling glycine and quantum dots to prepare the probes, marking of living cell using the glycine modified quantum dot probes, imaging of living cell and tracing, and the like. According to the invention, the quantum dots can hardly penetrate cell membranes, the probes used for the imaging of living cell are coupled with other molecules so as to enter the cell, and comparatively available coupling molecules are cell-penetrating peptide. The water-soluble quantum dots and the glycine are coupled to improve the cell-penetrating ability of the quantum dots, so as to allow the quantum dots in the cell to play the role of marking and tracing the cells. The glycine modified quantum dots are uniformly distributed in cytoplasm and are uniform and dispersed in shining, and the non-modified quantum dots are mainly attached on the cell membranes, and are comparatively large in particle and not uniform compared with the cell-penetrating peptide modified quantum dots. Besides, the glycine modified quantum dot probe has the advantages of low cost, quickness in manufacturing, stable quality, convenience in use, uniform cell-in distribution and the like.

Description

technical field [0001] The invention relates to a method for marking live cells with a glycine-modified quantum dot probe. Background technique [0002] Live cell imaging technology can observe the dynamic changes of cell internal structure and cell physiological process in real time for a period of time, while the small animal live imaging system can monitor the activity and gene behavior of cells in living organisms in real time. Research plays a vital role. [0003] The use of ideal fluorescent probes to label molecules or cells of interest is a necessary prerequisite for the smooth operation of live cell imaging techniques and small animal live imaging systems. Commonly used fluorescent probes include fluorescent proteins, small molecule organic fluorescent dyes, and quantum dots. Fluorescent protein molecules are relatively large, which is not suitable for some research; small molecule fluorescent dyes and quantum dot inorganic nanocrystalline particles (less than 10n...

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Application Information

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IPC IPC(8): G01N21/63G01N21/64
Inventor 胡炯宇韩健黄跃生
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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