Active mold inhibitor, as well as preparation method and application thereof
A mold and preparation technology, applied in the field of applied microorganisms, can solve the problem that there are not many reports on active mold preparation research, and achieve the effects of short cycle, high vitality and convenient storage.
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Embodiment 1
[0025] Embodiment 1: Active mold preparation is prepared by the following method:
[0026] (1) Inoculation and fermentation conditions: 1% inoculation amount of spore liquid, 200r / min vibration, 26°C for 40 hours, inoculated in 10L fermenter according to 3%-5% inoculum amount, fermentation medium components and first-grade seeds The composition of the culture medium is the same. At a temperature of 29°C and a ventilation ratio of 1:1, after 48 hours of cultivation, the fermentation broth is vacuum-filtered through a 200-mesh nylon filter cloth frame to obtain wet cells.
[0027] (2) Dry protection agent treatment: The dry protection agent is compounded with soluble starch, soybean powder, and glycerin. The compound ratio is 1:0.1:0.02, 121 ° C, 20 minutes of sterilization treatment, and the water is 121 ° C. Sterilize for 20 minutes and wait for use;
[0028] (3) Drying process: the above-mentioned sterilized water and drying aid protection agent are mixed according to the ma...
Embodiment 2
[0030] Embodiment 2: Active mold preparation is prepared by the following method:
[0031] (1) Inoculation and fermentation conditions: 5% inoculation amount of spore liquid, shaking at 220r / min, cultured at 29°C for 36 hours, then inoculated in a 10L fermenter according to 3% inoculum amount, the composition of the fermentation medium and the primary seed medium The ingredients are the same, at a temperature of 29°C and a ventilation ratio of 1:0.8, after 48 hours of cultivation, the fermentation broth is vacuum-filtered through a 300-mesh nylon filter cloth frame to obtain wet cells;
[0032] (2) Treatment of dry protection agent: The dry protection agent is compounded with soluble starch, soybean powder and glycerin, the compound ratio is 1:0.1:0.02, sterilized at 121°C for 20 minutes for use, water is passed through 121°C, Sterilize for 20 minutes and wait for use;
[0033] (3) Drying process: the above-mentioned sterilized water and drying aid protection agent are mixed ...
Embodiment 3
[0036] Embodiment 3: Active mold preparation is prepared by the following method:
[0037] (1) Inoculation and fermentation conditions: 10% inoculation amount of spore liquid, 200r / min shaking, 36-40 hours of cultivation at 29°C, inoculation in 10L fermenter according to 3%-5% inoculum amount, fermentation medium components and a The ingredients of the seed culture medium are the same. At a temperature of 29°C and a ventilation ratio of 1:1.2, after 48 hours of cultivation, the fermentation broth is vacuum-filtered through a 300-mesh nylon filter cloth frame to obtain wet cells;
[0038] (2) Drying aid and protection agent treatment: the drying aid and protection agent is compounded with soluble starch, soybean powder, and glycerin, sterilized at 121°C for 20 minutes for use, and water is sterilized for 20 minutes at 121°C for use;
[0039] (3) Drying process: the above-mentioned sterilized water and drying aid protection agent are mixed according to the mass ratio of 1:1 to f...
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