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Lactobacillus plantarum and micro-ecological preparation for silage

A technology of Lactobacillus plantarum and micro-ecological preparations, which is applied in the field of fermenting microorganisms and silage, can solve the problems such as the limited variety of micro-ecological preparations, and achieve the effects of shortening the fermentation cycle, good wall-breaking effect, and improving extraction rate

Active Publication Date: 2014-06-25
云南立达尔生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It should be noted that whether it is additive silage or mixed silage, most of the research is on common feed ingredients such as corn and alfalfa, while there are very few researches on marigold silage, which is dedicated to the microecology of marigold silage. There are not many types of preparation products

Method used

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  • Lactobacillus plantarum and micro-ecological preparation for silage

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Embodiment Construction

[0029] Isolation of strains:

[0030] Separation source: In September 2011, a certain weight of silage marigolds was taken from the marigold fermentation pond in Qinggang County, Heilongjiang Province;

[0031] Culture medium: MRS medium;

[0032] Separation and screening: Add 25g of silage marigolds into 225mL of deionized water (contains some glass beads), shake and shake well to obtain 10 -1 Diluent, let it stand, take the supernatant and dilute to 10 -2 、10 -3 、10 -4 、10 -5 . Take 0.1mL of each gradient and smear the plate (MRS), each with 3 replicates, and cultivate them in a constant temperature incubator. The strains were isolated and purified, and stored at 4°C for future use.

[0033] The morphological characteristics of strain Lder01 are as follows:

[0034] The colonies on the culture medium are convex lens-like protrusions with a diameter of 0.5-1.0mm, smooth surface, uniform texture, round shape, light yellow color, and have certain stickiness when picki...

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Abstract

The invention discloses a lactobacillus plantarum and a micro-ecological preparation for silage. The invention provides the lactobacillus plantarum Lder01 separated from a marigold fermentation tank and the lactobacillus plantarum-containing micro-ecological preparation for silage. The viable count of the lactobacillus plantarum Lder01 in the fermentation liquid can reach 9 billion colony forming units (CFU) per milliliter and reaches the leading level; the survival rate of the micro-ecological preparation for silage, which is preserved for 120 days at the temperature of 4 DEG C, is 100 percent, and the problem about the shelf life of the preparation is solved; and by using the micro-ecological preparation for silage, the fermentation period of fresh marigold can be effectively shortened, and the extraction rate of lutein is improved.

Description

technical field [0001] The invention belongs to the field of fermenting microorganisms and silage, and in particular relates to a strain of Lactobacillus plantarum and a microecological preparation for silage. Background technique [0002] Silage refers to the use of soluble carbohydrates in raw materials by lactic acid bacteria attached to plants under anaerobic conditions, and anaerobic fermentation produces organic acids (mainly lactic acid), resulting in a decrease in pH, thereby killing various microorganisms or inhibiting Its activity achieves the purpose of preserving green feed. According to the effect on the fermentation effect, silage additives can be divided into four categories: 1) fermentation-promoting additives, mainly including lactic acid bacteria, cellulase, glucose, and molasses; 2) fermentation-inhibiting additives, including sulfuric acid, hydrochloric acid, formic acid, acetic acid , formaldehyde, etc.; 3) nutritional additives, including urea, ammonia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K3/03C12R1/25
Inventor 陶正国洪梅吴秀丽孙丹丹
Owner 云南立达尔生物科技有限公司
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