Special lactobacillus inoculum for silage and its making method

The technology of lactic acid bacteria and inoculant is applied in the preservation method, bacteria and application of animal feed raw materials, which can solve the problems of high loss rate, high cost and high commodity cost, and achieve the effect of improving quality

Inactive Publication Date: 2006-06-21
王建华 +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The most widely used lactic acid bacteria abroad include Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus xylosus, Pediococcus pentosaceus, Pediococcus acidilactici, Propionibacterium shermanii, etc. The carbon source of the main components of the fermentation medium is generally glucose, lactose, soluble starch and sucrose, and the nitrogen source is generally whey powder, yeast culture and hydrolyzed animal protein. The fermentation products of batch fermentation are generally separated by filtration, ultrafiltration or centrifugation. Concentration; the isolated high-purity bacteria are either spray-dried (high loss rate) or freeze-dried (high cost), the latter requires a corresponding freeze-drying protective agent, except that the freeze-drying equipment mainly comes from a one-time investment The protective agent is the key to reduce the loss of live bacteria freeze-drying and prolong the shelf life of the product. The protective agent is mainly composed of skimmed milk powder or maltodextrin, glycerin, calcium carbonate, sucrose, maltose, sodium glutamate, inositol, manganese sulfate, etc.; Foreign SIB products such as SIL-ALL from the UK and Sil-Early from the United States guarantee the total number of live lactic acid bacteria > 10 billion CFU / ton (silage), and the total number of live lactic acid bacteria from LAB produced by AgMaster in Canada is guaranteed to be 100,000-600,000 CFU / ton (silage alfalfa, corn, etc.), so the total number of viable lactic acid bacteria on the market is generally 100-100 million CFU / g, and the shelf life is 1 year. Generally, frozen storage is used, and the cost of the product is relatively high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Special lactobacillus inoculum for silage and its making method
  • Special lactobacillus inoculum for silage and its making method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0057] Embodiment 2: product Lactobacillus plantarum of the present invention neutralizes Pediococcus pentosaceae count

[0058] 1. Medium

[0059] (1) 1% sterile peptone buffer solution: Accurately weigh 5 g of peptone and dissolve in 500 ml of deionized water, dispense into conical flasks, place in a sterilizer, sterilize at 121.5°C for 30 minutes, and set aside.

[0060] (2) MRS (Mgnn, Rogosa, Sharpe) medium composition and preparation (g / l): beef peptone 10g, yeast extract powder 5g, beef extract 10g, glucose 20g, Tween 80 1.0g, ammonium citrate 2.0g, acetic acid Sodium 5.0g, magnesium sulfate 0.1g, manganese sulfate 0.05g, dipotassium hydrogen phosphate 2.0g, deionized water 1000ML, pH=6.1-6.3, temperature 25°C. The pH of the medium was adjusted by ammonia water. According to the above formula, the reagent was weighed and dissolved in 1000ml deionized water, stirred and then packed in conical flasks. Then, sterilize at 121.5°C for 30-40 minutes, and when the temperature...

Embodiment 3

[0072] Embodiment 3, the influence of lactic acid bacteria inoculant of the present invention on corn silage quality and dairy cow production performance

[0073] 1. The silage inoculant of the present invention

[0074] The amount of silage used per ton is 10 grams, and the dry powdered inoculant is completely dissolved in water according to the ratio of 5 grams per liter, and then the SIB-HA inoculant is sprayed into the silo with a motorized knapsack sprayer. On the crushed silage layer, after each layer of silage is laid, the inoculant solution is sprayed once, compacted back and forth with a crawler tractor, and other links are treated according to conventional silage measures, which will be used for the feeding test of dairy cows in the next year.

[0075] 2. Silage quality assessment

[0076] Fresh whole-plant corn (spring corn variety Zhongyu No. 4) was chopped with a cutting machine at a dairy farm in the northern suburbs of Beijing, and the length was less than 50 mm....

Embodiment 4

[0096] The test of embodiment 4 dairy cow production performance

[0097] During the first 30 days of the experiment, the silage of the SIB-HA treatment group and the control silage had no effect on the milk production of dairy cows. After 30 days, the effects of treated silage and control silage on milk production of cows began to be evident. The results of the dairy cow feeding experiment showed that the daily milk yield of dairy cows was 0.9kg / head higher than that of the control under the treatment of SIB-HA. As a result, the annual milk production increased by 283 kg / head (the average lactation period was 305 days). In addition, based on the average daily intake of 20 kg of silage per cow, 1 ton of silage corn treated with SIB-HA can increase the milk yield by 46.5 kg due to the improvement of quality. This study also observed improved milk production in cows fed SIB-HA-treated corn silage for 30 days. Apparently, although the milk quality was not significantly improve...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

An inoculating lactic ferment for silage is prepared from two kinds of dried living bacteria powder, cellulase, semi-cellulase, carbohydrate, manganese sulfate and diatomite through medium-temp. pressure-reduction drying and gelatin-starch dual-coating or freeze-drying. Its advantages are great number of living bacteria 90 billions CFU / g, long storage period, and high effect (increasing milk by 283 kg).

Description

Technical field: [0001] The invention belongs to the field of feed processing, in particular to a special lactic acid bacteria inoculant for silage and a manufacturing method thereof. technical background: [0002] The dairy cattle industry in my country has developed rapidly. The output has increased by 5 times in the past 5 years. By 2000, it will reach 4.89 million heads (including adult fine breed cattle, improved cattle, young cattle and dual-purpose cattle for milk and meat production). The total milk output is 8.27 million tons. The average annual milk consumption per person is actually nearly 7 kilograms. If the average daily milk consumption per person is only 0.25 kilograms, the average annual milk consumption per person is nearly 81 kilograms. There is still room for development of more than 10 times the current basic level. It can be predicted that my country's dairy cows will develop in It will maintain a strong momentum for quite a long time, but what is not comp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A23K3/02A23K3/03C12N1/20A23K10/18A23K20/163A23K20/189A23K20/20A23K30/15A23K30/18A23K50/10
Inventor 王建华时建忠刁其玉张帆刘立恒滕达吴子林李桂杰
Owner 王建华
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products