Method for breeding and purifying edible mushroom strains at test tube medium-less and medium critical points

A technology based on critical point and purification method, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of unstable strain quality and complicated process, achieve effective purification, simple process, and maintain good quality Effect

Inactive Publication Date: 2014-04-09
LIUZHOU TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a new test-tube production process of edible fungus strains, which overcomes the shortcomings of traditional techniques such as unstable quality of strains and very complicated processes.

Method used

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Embodiment Construction

[0018] Below in conjunction with embodiment the method of the present invention is further described.

[0019] The test-tube baseless and base-based critical point breeding and purification method of edible fungus strains has the following process steps:

[0020] 1. Tissue separation: Select good and robust fruiting bodies of edible fungi, and cut 0.5-1 cm long meat tissue at the junction of the fruiting body cap and the stipe under a sterile environment.

[0021] 2. Test tube culture: place the meat tissue in a blank PDA slant test tube, and there are 2 to 5 mm above the slant culture medium, with the base-free and basal critical points.

[0022] 3. Mycelia cultivation: Place the inoculated test tubes at a temperature of 20-25°C and a relative humidity of 75-80% to cultivate mycelia.

[0023] 4. Transfer tube purification: After the mycelium grows to contact the slant medium, pick the rapidly growing and vigorous mycelium in a sterile environment and transfer it to a blank P...

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Abstract

The invention relates to the technical field of biology, in particular to a method for breeding and purifying edible mushroom strains at test tube medium-less and medium critical points. The method comprises the following steps of putting edible mushroom sporocarp meat loaf tissues which are separated at medium-less and medium critical points which are arranged on a blank tube and are 2-5 mm far from the side upper part of a slant culture medium, firstly sprouting hypha under medium-less environment, purifying the hypha in a rapid and strong growth state after the hypha contacts the culture medium, effectively detoxifying, and keeping the stable excellent characteristic of the strains. According to the method disclosed by the invention, the manufacturing process of the strains is simple in technology, the applicability is strong, the method is suitable for tissue separating and breeding of most of edible mushroom strains, and the success rate can be up to 99%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for breeding and purifying edible fungus strains in test tubes without bases and with bases. Background technique [0002] The production of edible fungi in my country still has a tendency of "emphasizing output and ignoring quality", resulting in the lack of competitiveness of some edible fungus products in the market. At the same time, the scientific research force in the breeding of bacteria is relatively weak, so that too much subculture of the bacteria will lead to the degradation of the vitality of the bacteria, and the quality of the bacteria will directly affect the output and quality of edible fungus products. Paying attention to the breeding of high-quality, high-yield, disease-resistant, insect-resistant and other excellent strains is the only way to ensure the production and quality of edible fungi, and it is also an important link to improve the prod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04A01H4/00
Inventor 何寒
Owner LIUZHOU TEACHERS COLLEGE
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