Cryopreservation liquid and cryopreservation method for adipose tissue-derived mesenchymal stem cells ( ADSC)

A technology of mesenchymal stem cells and cryopreservation solution, which is applied in the field of adipose-derived mesenchymal stem cell cryopreservation solution and its preparation, can solve the problems of lack of adipose-derived mesenchymal stem cell cryopreservation solution and unsatisfactory cryopreservation effect, so as not to affect the cells Growth characteristics, effects of high recovery rate

Active Publication Date: 2013-05-01
冯文峰
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Most of the existing mesenchymal stem cell cryopreservation solutions contain fetal bovine serum or DMSO, even if they do not contain fetal bo

Method used

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  • Cryopreservation liquid and cryopreservation method for adipose tissue-derived mesenchymal stem cells ( ADSC)
  • Cryopreservation liquid and cryopreservation method for adipose tissue-derived mesenchymal stem cells ( ADSC)
  • Cryopreservation liquid and cryopreservation method for adipose tissue-derived mesenchymal stem cells ( ADSC)

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Embodiment 1

[0034] A fat-derived mesenchymal stem cell cryopreservation solution, consisting of the following components in the following contents:

[0035]

[0036] Prepare adipose-derived mesenchymal stem cell cryopreservation solution according to the above ratio and composition. The adipose-derived mesenchymal stem cell cryopreservation liquid is cryopreserved by the following method.

[0037] 1) Aseptically absorb the fat extract from liposuction, wash it several times with PBS to remove the drugs and blood cells used in liposuction; digest with 0.1% type I collagenase at 37°C for 1 hour, centrifuge at 400g / min for 5min, and draw After the upper layer of fat, mix well and filter with a 200-mesh sieve; centrifuge the obtained cell suspension at 1200r / min for 8min, wash the cell pellet with PBS, and adjust the cell concentration to 1×10 9 cells / L, suspend in DMEM-LG medium containing 10% autologous serum by volume fraction, inoculate in 10 cm culture dish, place at 37°C, volume fra...

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Abstract

The invention relates to a cryopreservation liquid and a cryopreservation method for adipose tissue-derived mesenchymal stem cells ( ADSC). The cryopreservation liquid is composed of the following materials: 3 to 10 mg/ml of vitamine C, 2 to 8 mg/ml of vitamin E, 2 to 8 mg/ml of lipoic acid, 5 to 20 volume percent of human autoserum, and DMEM-LG culture medium in balancing amount. The cryopreservation liquid for ADSC does not contain animal serum and DMSO, and is composed of human autoserum, lipoic acid, vitamin E, vitamin C and other main materials. A cell cryopreserved with the cryopreservation liquid has the advantages of high palinesthesia rate, no impact on cell growth characteristic, and maintanience of stem cell pluripotency after palinesthesia.

Description

technical field [0001] The invention relates to a cryopreservation liquid for stem cells, in particular to a cryopreservation liquid for adipose-derived mesenchymal stem cells and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells were originally discovered in the bone marrow, and have attracted increasing attention because of their multi-lineage differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. Under specific induction conditions in vivo or in vitro, mesenchymal stem cells can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, endothelial and other tissue cells. It has multi-directional differentiation potential and can be used as an ideal seed cell for the repair of t...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 冯文峰佘志勇方海庆
Owner 冯文峰
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