Mosquito-killing gel and preparation method thereof
A mosquito gel and gel technology, which are applied in the field of mosquito-killing gel, can solve the problems of difficulty in meeting practical application, poor vitality, etc., and achieve the effects of good mosquito-killing activity, long duration and simple production process
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Embodiment 1
[0027] The preparation of embodiment 1 mosquito-killing gel of the present invention
[0028] a. Take 200g of Pythium sp.GY1938 mycelium;
[0029] b. Take 1 / 4KPYG 2 Medium (composition: 0.375g peptone, 0.125g yeast extract, 0.75g glucose, 6.25mg cholesterol, 27.5mg calcium chloride, 2.5g corn oil, add water to 1L), add 10g trehalose, adjust the pH value to 7.0~10.0 , add 1g of agar powder, melt, cool to 60°C, add mycelia, homogenate and stir to obtain a gel suspension, put it in a mold immediately, cut into pieces after solidification, and package and store at 4-8°C.
Embodiment 2
[0030] Embodiment 2 The preparation of mosquito-killing gel of the present invention
[0031] a. Take 300g of Pythium sp.GY1938 mycelium;
[0032] b. Take 1 / 2KPYG 2 Culture medium (composition: 0.75g peptone, 0.25g yeast extract, 1.5g glucose, 12.5mg cholesterol, 55mg calcium chloride, 5g corn oil, add water to 1L), adjust the pH value to 7.0~10.0, add 2g agar powder, dissolve , cooled to 40°C, added mycelium, homogenized and stirred to obtain a gel suspension, which was immediately placed in a mold, solidified, cut into pieces and shaped, packaged and stored at 4-8°C.
Embodiment 3
[0033] Embodiment 3 Preparation of mosquito-killing gel of the present invention
[0034] a. Take 150g of Pythium sp.GY1938 mycelium;
[0035] b. Take 1 / 8KPYG 2 Culture medium (composition: 0.1875g peptone, 0.0625g yeast extract, 0.375g glucose, 3.125mg cholesterol, 13.75mg calcium chloride, 1.25g corn oil, add water to 1L), add 25g trehalose, adjust the pH to 7.0~10.0 , add 0.5g of agar powder, melt, cool to 60°C, add mycelium, homogenate and stir to obtain a gel suspension, put it in a mold immediately, cut into pieces after solidification, and package and store at 4-8°C.
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