Method for preparing water soluble chitosan oligosaccharide

A technology of water-soluble chitosan and chitosan, which is applied in the preparation of sugar derivatives, chemical instruments and methods, amino sugars, etc., can solve the problems of incomplete degradation process, waste of raw materials, high risk, etc., and achieve microwave degradation The effect of complete degree, reduction of process cost, and shortening of radiation time

Inactive Publication Date: 2013-05-01
SHANGHAI FENGYU BIOLOGICAL TECH DEVCO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a method for preparing water-soluble chitosan oligosaccharid

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  • Method for preparing water soluble chitosan oligosaccharide

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[0013] Example 1

[0014] After preliminary treatment, 1kg of the raw material polymer chitosan is soaked in pure water for 6h to form 1000ml of a mixture of chitosan and pure water, and then the soaked mixture is placed on a microwave generator for tunnel continuous microwave The treatment can ensure that the soaked chitosan receives uniform microwave radiation. The microwave radiation time is 5min and the microwave radiation temperature is 130℃; the solution obtained after microwave radiation is degraded with 3000ml of food-grade hydrogen peroxide. Distilled water is added during the degradation process of food grade hydrogen peroxide. After the reaction is complete, the nano-membrane filtration separates the crude chitosan and the filtrate with low molecular weight. The filtrate can be sent to the soaking process for reuse; the crude chitosan is washed with pure water to remove impurities , The washed solution is centrifuged and concentrated at high speed, the concentrated pro...

Example Embodiment

[0015] Example 2

[0016] After preliminary treatment, 1 kg of the raw material polymer chitosan is soaked in pure water for 8 hours to form 1000 ml of a mixture of chitosan and pure water, and then the mixture obtained after soaking is placed on the microwave generator for continuous tunneling Microwave treatment can ensure uniform microwave radiation to the soaked chitosan. The microwave radiation time is 30min and the microwave radiation temperature is 120℃; the solution obtained after microwave radiation is degraded with 2500ml of food-grade hydrogen peroxide. , Distilled water is added during the degradation of food-grade hydrogen peroxide. After the reaction is complete, the nano-membrane filtration separates the crude chitosan and the filtrate with low molecular weight. The filtrate can be sent to the soaking process for reuse; the crude chitosan is washed and removed with pure water For impurities, the washed solution is centrifuged and concentrated at high speed, the con...

Example Embodiment

[0017] Example 3

[0018] After preliminary treatment, 1 kg of the raw material polymer chitosan is soaked in pure water for 10 hours to form 1000 ml of a mixture of chitosan and pure water, and then the mixture obtained after soaking is placed on the microwave generator for continuous tunneling Microwave treatment can ensure that the soaked chitosan receives uniform microwave radiation. The microwave radiation time is 60min and the microwave radiation temperature is 100℃; the solution obtained after microwave radiation is degraded with 2000ml of food-grade hydrogen peroxide. , Distilled water is added during the degradation of food grade hydrogen peroxide. After the reaction is complete, the low molecular weight chito-oligosaccharides and filtrate are separated by nano-membrane filtration. The resulting filtrate can be sent to the soaking process for repeated use; the chito-oligosaccharides are washed with pure water Remove impurities, centrifuge and concentrate the washed solut...

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Abstract

A method for preparing water soluble chitosan oligosaccharide comprises the following steps: placing preliminarily treated raw material high molecular weight chitosan into a pure water and then placing a soaked mixed solution in a microwave generator so as to carry out tunnel type continuous microwave treatment on the mixed solution; degrading the obtained mixed solution treated by microwaves through using food-grade hydrogen peroxide,; and obtaining low molecular weight chitosan crude product and a filter liquor through nano membrane separation, wherein the filter liquor is fed into an initial soaking step to be continuously used and the chitosan crude product is purified to obtain a chitosan finished product. The method for preparing the water soluble chitosan oligosaccharide, provided by the invention, has the advantages as follows: due to the adoption of the tunnel type continuous microwave treatment, the chitosan soaked in the solution is uniformly radiated, so that the microwave radiation time is reduced and the microwave degradation degree is completer; and as a nano membrane filtering method replaces an alcohol precipitation method in the purifying process, potential threats caused by storage and the use of a great deal of ethanols are avoided and the technical cost is also reduced.

Description

technical field [0001] The invention belongs to the field of degradation of macromolecular sugars, in particular to a method for preparing water-soluble chitosan oligosaccharides. Background technique [0002] Chitosan oligosaccharide is also called chitosan oligosaccharide, also known as chitosan oligosaccharide, scientific name β-1,4-oligosaccharide-glucosamine, is a brand new product obtained by processing chitosan, and has good water solubility , Low molecular weight product with great function and high biological activity. It has many unique functions such as high solubility and easy absorption by organisms that chitosan does not have, and its effect is 14 times that of chitosan. [0003] At present, the preparation methods of chitosan oligosaccharide mainly include chemical method, enzymatic method and physical method. Among them, chemical methods mainly include acid degradation method and oxidative degradation method. Functional groups attack many free amino groups ...

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Application Information

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IPC IPC(8): C07H5/06C07H1/00C08B37/08
Inventor 谷经建
Owner SHANGHAI FENGYU BIOLOGICAL TECH DEVCO LTD
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