Lactobacillus crispatus and application

A technology of Lactobacillus crispatus and Pseudomonas aeruginosa, applied in bacteria, antibacterial drugs, medical raw materials derived from bacteria, etc., can solve the problems of aggravating vaginal microecological disorders, repeated BV recurrence, etc., and achieve the effect of strong acid resistance

Inactive Publication Date: 2013-05-01
SHANGHAI JIAODA ONLLY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the application of antibiotics can temporarily relieve the symptoms of BV, it also further reduces the alr...

Method used

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  • Lactobacillus crispatus and application
  • Lactobacillus crispatus and application
  • Lactobacillus crispatus and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Microscopic examination of bacterial strains

[0018] Under sterile conditions, Lactobacillus crispatus LCR15 was picked with an inoculation loop, spread on a glass slide, prepared and stained with Gram, and observed the morphology of the bacteria under a microscope.

Embodiment 2

[0019] Embodiment 2: MRS plate purification separation

[0020] Pick Lactobacillus crispatus LCR15 with an inoculation loop under sterile conditions, streak on the MRS plate, place the plate in an incubator at 37°C for 48 hours, and select a single colony for microscopic examination to achieve pure strain isolation.

Embodiment 3

[0021] Embodiment 3: strain fermentation

[0022] Use an inoculation loop to pick two rings of Lactobacillus crispatus LCR15 from the slope to 15ml of MRS liquid medium, culture at 37°C for 24h, then insert 1% inoculum into 15ml of MRS liquid medium, and culture at 37°C for 24h. The fermentation broth of Lactobacillus crispatus LCR15 was obtained.

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Abstract

The invention discloses a Lactobacillus crispatus and an application. The Lactobacillus crispatusis is preserved in the China General Microbiological Culture Collection Center (CGMCC) with accession number of CGMCC NO.6406. The Lactobacillus crispatus has high acid resistance, high-yield hydrogen peroxide characteristic and vagina pathogenic bacteria growth inhibition effect, and the invention also relates to an application of the Lactobacillus crispatus LCR15 for adjusting vaginal flora, preventing and treating colpitis.

Description

technical field [0001] The present invention relates to a kind of new bacterial strain of Lactobacillus and application thereof, more precisely, the present invention has described Lactobacillus crispatus ( Lactobacillus crispatus ) of a new strain LCR15, and its high acid resistance, high hydrogen peroxide production characteristics, antibacterial effect on vaginal pathogenic bacteria and the function of prevention and treatment of vaginitis. Background technique [0002] There are a variety of microorganisms in the vagina of healthy women, and they form a vaginal micro-ecological system that restricts, coordinates and dynamically balances with the host and the environment. The vaginal flora of healthy women is mainly composed of Lactobacillus, including Lactobacillus crispatus, Lactobacillus jansneri and Lactobacillus gasseri. Under normal circumstances, Lactobacillus can protect the vagina, but the disturbance of vaginal microecology in which Lactobacillus dominates can ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/04C12R1/225A61K35/747
CPCY02A50/30
Inventor 张旻杭晓敏张和春夏祎稚陈培青
Owner SHANGHAI JIAODA ONLLY CO LTD
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