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Oxytropis medical material and detection method of oxytropis medical material preparation

A technology of detection method and preparation method, which is applied in the direction of measurement device, material separation, analysis material, etc., can solve the problem of lack of quantification and other index control, and achieve the effect of strong specificity, accurate results and good reproducibility

Active Publication Date: 2014-12-17
GANSU CHEEZHENG TIBETAN MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The Oxytropis medicinal material and its preparations are widely used in Tibetan medicine, but the original standard only has its detection methods such as source, character, microscopic qualitative, etc., and the detection method is relatively simple, lacking quantification and other index control
There is no report on the TLC identification and content detection method of the Oxytropis medicinal material and its preparations in the present technology

Method used

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  • Oxytropis medical material and detection method of oxytropis medical material preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0043] Experimental example 1: TLC Identification of Oxytropis

[0044] (1) Instrument

[0045] Electronic scales, Erlenmeyer flasks with stoppers, pipettes, ultrasonic extractors, filter papers, spotters, silica gel G-plates, chromatography cylinders, UV lamps, spray bottles.

[0046] (2) Reference substance

[0047] 2′,4′-Dihydroxychalcone, with a purity of 98%, was purchased from Alfa Corporation of the United States.

[0048] (3) Reagents

[0049] Methanol, ethanol, formic acid, ethyl acetate, petroleum ether, n-hexane.

[0050] (4) Inspection method:

[0051] Extraction solvent: ethanol, methanol, 80% methanol, ethyl acetate.

[0052] Extraction method: shaking and ultrasound.

[0053] Developing solvent: petroleum ether (60-90°C)-n-hexane-ethyl acetate (7:4:3), ethyl acetate-chloroform-petroleum ether (60-90°C) (7:2:3).

[0054] The above extraction solvents, extraction methods and developing agents were respectively selected for testing, and the results are as ...

experiment example 2

[0059] Experimental Example 2: Determination of the content of Oxytropis 2′,4′-dihydroxychalcone contained in Oxytropis medicinal material

[0060] (1) Instrument

[0061] Electronic balance, Erlenmeyer flask, volumetric flask, pipette, syringe, beaker, funnel, filter paper, filter head, high performance liquid chromatography.

[0062] (2) Reference substance

[0063] 2′,4′-Dihydroxychalcone, with a purity of 98%, was purchased from Alfa Corporation of the United States.

[0064] (3) Reagents

[0065] Methanol, acetonitrile, ethanol, absolute ethanol, formic acid, water.

[0066] (4) Inspection method:

[0067] Extraction solvent: absolute ethanol, 70% ethanol, 30% ethanol.

[0068] Extraction time: ultrasonic 10 minutes, 40 minutes, 60 minutes.

[0069] Mobile phase: methanol-water (30:70), acetonitrile-0.1% aqueous formic acid (60:40)

[0070] The above extraction solvent, extraction time and mobile phase were respectively selected for testing, and the results are a...

experiment example 3

[0075] Experimental example 3: Determination of the content of 2′,4′-dihydroxychalcone in Oxytropis chinensis—methodological examination observe

[0076] (1) Instruments and reagents

[0077] Agilent 1100 high-performance liquid chromatograph (Agilent Corporation of the United States): G1314A ultraviolet-visible detector, G1314A binary pump, Agilent1100 chromatographic workstation. SK8200HP ultrasonic cleaner (power 250W, frequency 40kHz) (Shanghai Kedao Ultrasonic Instrument Co., Ltd.).

[0078] 2′,4′-Dihydroxychalcone reference substance (purchased from Alpha Company of the United States); Oxytropis chinensis medicinal material (provided by Gansu Qizheng Tibetan Medicine Co., Ltd.); acetonitrile (chromatographically pure); methanol (analytical pure); formic acid ( Chromatographically pure).

[0079] (2) Chromatographic conditions

[0080] Octadecyl bonded silica gel is used as filler; Chromatographic column: Agilent C18 (4.6×250mm, 5.0μm, Agilent Corporation);

[008...

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Abstract

The invention relates to an oxytropis medical material and a detection method of oxytropis medical material preparation. The detection method comprises the steps of one or a plurality of the following identification or detection method(s): qualitatively identifying oxytropis of the oxytropis medical material or the oxytropis medical material preparation by a thin-layer chromatography; and qualitatively identifying the content of 2', 4'-dihydroxy chalcone in the oxytropis medical material or the oxytropis medical material preparation by a high-performance liquid chromatography. Effective detection of the oxytropis medical material or the oxytropis medical material preparation can be realized by adopting the detection method.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a detection method of medicinal materials and preparations thereof. Background technique [0002] According to the 78th edition of "Tibetan Medicine Standards" in six provinces and regions: Oxytropis is the dried whole plant of Oxytropis chiliophylla Royle or Oxytropis falcata Bunge of the leguminous family. Its source is the same as that of the appendix of "Chinese Pharmacopoeia" 2010 edition. It is cold in nature, bitter in taste, and poisonous. For the treatment of plague, poisoning disease, yellow water disease, constipation, anthrax; external application to treat sores, furuncles, swelling and pain. However, under the item of Oxytropis falciparum (Edaxia) in "Standards of Tibetan Medicine of Qinghai Province", the source is stipulated as "this product is the dried whole herb of Oxytropis falcata Bunge of the leguminous plant", which is cons...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/02
Inventor 李波魏学明张国霞王维波刘鸿雁陈丽娟
Owner GANSU CHEEZHENG TIBETAN MEDICINE CO LTD
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