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Determination method of hesperidin content

A method of determination, the technology of hesperidin, applied in the field of determination of hesperidin content, can solve the problems of complex processing, inability to remove impurities, and many impurities, achieve specificity and repeatability, facilitate quality control, and be simple and fast to operate Effect

Active Publication Date: 2013-05-08
JIANGSU ALAND NOURISHMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If this scheme is used for the determination of hesperidin in the mixture of citrus bioflavonoids, first, there are too many impurities in the test solution, and the isocratic elution cannot completely remove the impurities; The third is that the interference of vitamin C on the extraction of hesperidin is not considered, and this method is not completely suitable for the determination of hesperidin in citrus bioflavonoids and vitamin C compound preparations

Method used

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  • Determination method of hesperidin content

Examples

Experimental program
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Effect test

Embodiment 1

[0012] Example 1. Vitamin C and citrus bioflavonoid compound preparation

[0013] (1) Preparation of diluted solution: 20mmol / l sodium hydroxide solution and 50% methanol solution are mixed uniformly in a ratio of 1:1;

[0014] (2) Preparation of extraction solution: Mix 75mmol / l sodium hydroxide solution and 50% methanol solution in a ratio of 1:1;

[0015] (3) Preparation of reference solution: Accurately weigh an appropriate amount of hesperidin and naringin reference substances, add diluted solution to prepare a reference solution with a concentration of 0.05 mg / ml of hesperidin and naringin;

[0016] (4) Preparation of the test solution: Accurately weigh 600mg of the sample into a 100ml volumetric flask, add 80ml of extraction solution, ultrasonicate at 65°C for 15min, take it out, cool to room temperature, dilute to the mark with the extraction solution, and take part of the solution for use 0.45μm microporous membrane filtration and standby;

[0017] (5) Chromatograph...

Embodiment 2

[0028] Example 2. Compound preparation of vitamin C and citrus bioflavonoids Batch number: CT4Y123002

[0029] (1) Preparation of diluted solution: Mix 20mmol / l sodium hydroxide solution and 50% methanol solution in a ratio of 1.5:0.5;

[0030] (2) Preparation of extraction solution: Mix 75mmol / l sodium hydroxide solution and 50% methanol solution in a ratio of 0.9:1.1;

[0031] (3) Preparation of reference solution: Accurately weigh an appropriate amount of hesperidin and naringin reference substances, add diluted solution to prepare a reference solution with a concentration of 0.05 mg / ml of hesperidin and naringin;

[0032] (4) Preparation of the test solution: Accurately weigh 550mg of the sample into a 100ml volumetric flask, add 80ml of the extraction solution, ultrasonicate at 60°C for 20min, take it out, cool to room temperature, dilute to the mark with the extraction solution, and take part of the solution for use 0.45μm microporous membrane filtration and standby;

...

Embodiment 3

[0044] Example 3. Compound preparation of vitamin C and citrus bioflavonoids Batch number: CT4Y123003

[0045] (1) Preparation of diluted solution: Mix 20mmol / l sodium hydroxide solution and 50% methanol solution in a ratio of 0.5:1.5;

[0046] (2) Preparation of extraction solution: Mix 75mmol / l sodium hydroxide solution and 50% methanol solution in a ratio of 1.1:0.9;

[0047] (3) Preparation of reference solution: Accurately weigh an appropriate amount of hesperidin and naringin reference substances, add diluted solution to prepare a reference solution with a concentration of 0.05 mg / ml of hesperidin and naringin;

[0048] (4) Preparation of the test solution: Accurately weigh 650mg of the sample into a 100ml volumetric flask, add 80ml of extraction solution, ultrasonicate at 70°C for 15min, take it out, cool to room temperature, and dilute to the mark with the extraction solution. Take part of the solution and filter it with a 0.45 μm microporous membrane for later use; ...

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Abstract

The invention discloses a determination method of hesperidin content, which comprises the following steps of: (1) preparing a dilute solution; (2) preparing an extract solution; (3) preparing a reference solution; (4) preparing a test sample solution; (5) determining chromatographic conditions; and (6) detecting a program. The determination method of the hesperidin content disclosed by the invention is convenient, simple and fast to operate, good in specificity and repeatability, high in recovery ratio, can accurately determine the hesperidin content, and is convenient for controlling qualities of citrus bioflavonoids and vitamin C complex preparations.

Description

technical field [0001] The invention relates to the field of biological testing, in particular to a method for determining the content of hesperidin in a composite preparation of vitamin C and citrus bioflavonoids. Background technique [0002] Citrus bioflavonoids are a series of flavonoids with significant biological activity extracted from citrus fruits. Citrus flavonoids have unique aroma, good water solubility and significant pharmacological effects. The functions of citrus flavonoids are: 1. Regulate blood lipids, reduce blood viscosity, improve serum lipids, prolong red blood cell life and enhance hematopoietic function, and prevent cardiovascular and cerebrovascular diseases. 2. Inhibit the growth of HL-60 leukemia cells and dissolve cancer cells. 3. It can effectively remove free radicals and toxins in the body, prevent and reduce the occurrence of diseases. 4. Anti-inflammatory, anti-allergic, broad-spectrum antibacterial and antiviral effects. Vitamin C has ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 朱伟薛红芬
Owner JIANGSU ALAND NOURISHMENT
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