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58 results about "Folch solution" patented technology

Folch solution is a solution containing chloroform and methanol, usually in a 2:1 (vol/vol) ratio. One of its uses is in separating polar from nonpolar compounds, for example separating nonpolar lipids from polar proteins and carbohydrates in blood serum.

Methods of synthesizing cell-free protein

One embodiment of the present invention is a diffusion continuous batch cell-free protein-synthesis method characterized simultaneously by continuously supplying substrate and energy source molecules in the supply phase to the reaction phase by the free diffusion via interface between both phases and by transferring by-products formed in the reaction phase by enhancing the efficiency of the synthesis reaction by prolonging the reaction lifetime by directly contacting a synthesis reaction mixture (reaction phase) containing a biological extract with a substrate- and energy source-supplying solution (supply phase) without using barrier such as semi-permeable membrane or ultrafiltration membrane in a general cell-free protein-synthesis reaction means. Another embodiment of the present invention is a dilution batch cell-free protein synthesis method characterized by enhancing the efficiency of the protein synthesis by prolonging the reaction lifetime by adding a diluting solution to the reaction mixture after pre-incubating the reaction mixture in a cell-free protein-synthesis reaction means using a wheat-embryo extract. Another embodiment of the present invention is a method characterized by enhancing the efficiency of the synthesis reaction simultaneously by re-supplying substrate and energy sources necessary for the protein synthesis (e.g., amino acids, ATP, GTP, creatine phosphate) to the reaction mixture using a gel filtration column and / or semipermeable membrane and by discontinuously removing by-products formed during the reaction after the synthesis reaction stops in the batch cell-free protein synthesis method.
Owner:CELLFREE SCI

Hydrogenation catalyst carrier the concentrations of acid additives of which are distributed in gradient reducing and preparation method thereof,

The invention relates to a hydrogenation catalyst carrier the concentrations of acid additives of which are distributed in gradient reducing, and a preparation method thereof; the preparation method comprises the following steps: adopting Al2O3 or Al2O3 containing SiO2, TiO2 and ZrO2 as a carrier, preparing a solution of higher concentration of the acid additives, and saturating and spraying the carrier by gradually adding deionized water to dilute the solution in the process of soaking the carrier; or preparing at least two kinds of solutions of different concentrations of the acid additives, and then spraying the solutions on the carrier in a descending order of the concentration of the acid additives; then drying for 1-8 hours at 80-150DEG C, and then roasting for 2-6 hours in air at 300-650DEG C; the acid additives are compounds containing P, B or F; and the preparation is simple, the manufacturing cost is low, the carrier is used as a carrier for a heavy-oil hydrogenation catalyst, the preparation method is simple, the manufacturing cost is low, the acidity and pore properties of the carrier can be effectively modified, and the acidity of the catalyst can be effectively modified and / or the interaction between active components and the carrier is improved.
Owner:PETROCHINA CO LTD

Method for preparing strong acid type ion exchange fibers by sulfonating PP-ST-DVB fibers

The invention discloses a method for preparing strong acid type ion exchange fibers by sulfonating PP-ST-DVB fibers. The method comprises the following steps: swelling the PP-ST-DVB fibers by using a swelling agent for 6-12 hours; then adding a sulfonating solution into the bottom of a reaction kettle and gradually heating to finish a sulfonating reaction; after the sulfonating reaction is finished, recycling the sulfonating solution into a solution storage tank; then, removing the swelling agent attached on the surfaces of the fibers by reduced pressure distillation; collecting and refluxing the volatile swelling agent into the solution storage tank; and after reduced pressure and distillation, obtaining PP-ST-DVB-based strong acid type ion exchange fibers and subpackaging for storage so as to avoid acid gradient washing. A certain amount of a dilution solution of chlorosulfonic acid is supplemented in the sulfonating solution and the sulfonating solution can be circularly utilized, so that the PP-ST-DVB fibers are continually sulfonated to prepare the strong acid type ion exchange fibers. The sulfonating method has the advantages that the preparation process is simple, the cost is obviously reduced, the acid solution gradient solution is not needed, no waste solutions are discharged, the sulfonating solution can be circularly utilized and the like.
Owner:河南省科学院同位素研究所有限责任公司 +1

Isonaringin extraction and separation process

The invention provides an isonaringin extraction and separation process, which belongs to the technical field of plant extraction. The isonaringin extraction and separation process comprises the following steps: (1) carrying out reflux extraction on an extraction raw material by using an extraction agent to obtain a flavone concentrated solution; (2) adjusting the pH value of the flavone concentrated solution to 7.5-8.5 by using an inorganic acid, standing, carrying out suction filtration, and taking a mother solution, namely, a liquid containing isonaringin; (3) continuing to adjust the pH value to 5-7 with an inorganic acid, uniformly stirring, carrying out crystallizing, and carrying out suction filtration to obtain a crude isonaringin product; (4) adding the crude isonaringin product into a polar organic solvent, heating the organic solvent to dissolve the crude isonaringin product, carrying out filtering to remove insoluble impurities, carrying out concentrating and crystallizing,carrying out suction filtration, carrying out washing with water, and carrying out drying to obtain a semi-finished isonaringin product; and (5) dissolving the semi-finished isonaringin product witha polar organic solvent, stirring for dissolving, carrying out filtering to remove impurities, adding pure water to dilute the solution, stirring, standing for crystallization, carrying out suction filtration, carrying out washing with pure water to neutrality, and carrying out drying to obtain a yellow isonaringin product. Isonaringin is obtained through extraction, separation and refining, the content reaches up to 90% or above, and the purity is 95% or above.
Owner:YAAN TAISHI BIOLOGICAL SCI & TECH CO LTD
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