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Isonaringin extraction and separation process

A technology of isonaringin and process, which is applied in the field of extraction and separation technology of isnaringin, can solve the problems of no report on isonaringin extraction and separation process, no report on refining, etc., and achieves improved commercial value, low cost, raw material easy-to-get effect

Active Publication Date: 2020-04-17
YAAN TAISHI BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The above-mentioned patents and prior art generally describe the extraction and separation process of other flavonoids, but the extraction and separation process of isonaringin has not been reported, let alone refined Aspects of reports

Method used

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  • Isonaringin extraction and separation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045]Take young citrus fruit, dry until the water content is lower than 15%, crush it through a 30-mesh sieve after drying, take 200g of young citrus fruit powder, add 1200mL of 73% ethanol aqueous solution, then adjust the pH to 9 with 20% NaoH solution, and reflux at 60°C Extract for 2 hours, collect the filtrate by filtration, and then reflux the filtrate with 1000mL of 73% ethanol aqueous solution with a pH of 9 at 60°C for 2 times, each time for 1 hour, combine the filtrate three times for a total of 3000mL, recover ethanol with a rotary evaporator to obtain 600mL of flavonoids and concentrate liquid; the citrus flavonoids concentrated solution was adjusted to ph with hydrochloric acid to be 8, stirred evenly, left to stand for 3h and then suction filtered, and the mother liquor was taken, and continued to be adjusted to ph with hydrochloric acid to be 6.5, after stirring, crystallization for 8h and suction filtration to obtain 8g of isonaringin crude product (Crude produ...

Embodiment 2

[0048] Such as figure 1 , take young citrus fruit, dry until the water content is lower than 15%, crush it through a 30-mesh sieve after drying, take 200g of young citrus fruit powder, add 1200mL of 75% ethanol aqueous solution, and then adjust the pH to 9.5 with 20% NaoH solution; Extract under reflux for 2 hours, and collect the filtrate by filtration; the filter material is refluxed again with 1000 mL of 73% ethanol aqueous solution with a pH of 9.5 at 75 ° C for 2 times, each time for 1 hour, and the filtrates are combined for a total of 3100 mL, and the ethanol is recovered by a rotary evaporator to obtain 650 mL Concentrated flavonoids: use hydrochloric acid to adjust the ph of citrus flavonoids to 8, stir evenly, let stand for 3 hours, then filter with suction, take the mother liquor, continue to use hydrochloric acid to adjust the pH to 6.5, stir evenly, crystallize for 8 hours, and then filter with suction to obtain 6g of isopomelo peel Glycoside crude product (crude ...

Embodiment 3

[0055] Take young citrus fruit, dry until the water content is lower than 15%, crush it through a 25-mesh sieve after drying, take 200g of young citrus fruit powder, add 1200mL of 70% ethanol aqueous solution, then adjust the pH to 10 with 20% NaoH solution, and reflux at 75°C Extract for 2 hours, collect the filtrate by filtration, and then reflux the filtrate with 1000mL of 70% ethanol aqueous solution with a pH of 10 at 75°C for 2 times, each time for 1 hour, combine the filtrate three times for a total of 3000mL, recover ethanol with a rotary evaporator to obtain 600mL of flavonoids and concentrate liquid; the citrus flavonoid concentrated solution is adjusted to 7.5 with hydrochloric acid, stirred evenly, and suction filtered after standing for 3 hours, and the mother liquor was taken, and continued to be adjusted to 6.5 with hydrochloric acid, and the crystallization after stirring for 8 hours was obtained by suction filtration to obtain 8g of isonaringin crude product (C...

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Abstract

The invention provides an isonaringin extraction and separation process, which belongs to the technical field of plant extraction. The isonaringin extraction and separation process comprises the following steps: (1) carrying out reflux extraction on an extraction raw material by using an extraction agent to obtain a flavone concentrated solution; (2) adjusting the pH value of the flavone concentrated solution to 7.5-8.5 by using an inorganic acid, standing, carrying out suction filtration, and taking a mother solution, namely, a liquid containing isonaringin; (3) continuing to adjust the pH value to 5-7 with an inorganic acid, uniformly stirring, carrying out crystallizing, and carrying out suction filtration to obtain a crude isonaringin product; (4) adding the crude isonaringin product into a polar organic solvent, heating the organic solvent to dissolve the crude isonaringin product, carrying out filtering to remove insoluble impurities, carrying out concentrating and crystallizing,carrying out suction filtration, carrying out washing with water, and carrying out drying to obtain a semi-finished isonaringin product; and (5) dissolving the semi-finished isonaringin product witha polar organic solvent, stirring for dissolving, carrying out filtering to remove impurities, adding pure water to dilute the solution, stirring, standing for crystallization, carrying out suction filtration, carrying out washing with pure water to neutrality, and carrying out drying to obtain a yellow isonaringin product. Isonaringin is obtained through extraction, separation and refining, the content reaches up to 90% or above, and the purity is 95% or above.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to an extraction and separation process of isonaringin. Background technique [0002] Citrus (Citrus reticulata Blanco) belongs to the Rutaceae plant. Citrus young fruit and peel have high medicinal value, among which vitamin P flavonoids can expand coronary arteries, increase coronary blood flow, anti-inflammatory, anti-ulcer, antibacterial And choleretic and other effects. Such compounds include hesperidin, hesperetin, naringin, naringenin, polymethoxylated flavones, neohesperidin, isonaringin, and the like. Most of the early studies focused on the main components such as hesperidin and naringin. In recent years, the research on naringenin, polymethoxylated flavones, and isonaringin has been increasing. [0003] Isonaringin, also known as rutin naringin (narirutin), is a dihydroflavonoid compound. Studies have found that isonaringin binds to β-Secretase 1 to affect prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H17/07C07H1/08
Inventor 郭军伟李文霞杨军王顺尧
Owner YAAN TAISHI BIOLOGICAL SCI & TECH CO LTD
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