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Primer and method for fast polymerization of multiple resistant genes of tomato yellow leaf curl virus

A technology of tomato yellowing curved leaves and resistance genes, applied in the field of tomato breeding for breeding disease-resistant inbred lines, achieving the effects of low cost, saving time and manpower, and high specificity

Inactive Publication Date: 2013-05-15
ZHEJIANG UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is difficult to aggregate them into the same genotype using conventional techniques

Method used

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  • Primer and method for fast polymerization of multiple resistant genes of tomato yellow leaf curl virus
  • Primer and method for fast polymerization of multiple resistant genes of tomato yellow leaf curl virus
  • Primer and method for fast polymerization of multiple resistant genes of tomato yellow leaf curl virus

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Embodiment

[0059] (1) Identification method of yellow leaf curl virus disease (TYLCV) resistance

[0060] It is a preferred method to plant and inoculate local viruses in natural high-prone fields to identify plant resistance. Tomato seedlings are grown in the field through inoculation of artificial feeding with poisonous (TYLCV) Bemisia tabaci ( Bemisia tabaci ) Carry out natural inoculation. Observe the onset symptoms of the plants after inoculation in the field. The severity of the onset of each individual plant needs to be counted three times. The third statistical analysis data is used to evaluate the most accurate resistance level and reduce the possibility of escape. The symptoms of TYLCV virus disease are usually classified into 0-4 levels. Grade 0: Asymptomatic; Grade 1: The leaves are slightly yellow; Grade 2: The leaves are obviously yellow and curled, and part of the plant tissue is symptomatic; Grade 3: Plant growth is retarded, the leaves are severely yellow and curled, and ...

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Abstract

The invention discloses a primer and a method for fast polymerization of multiple resistant genes of a tomato yellow leaf curl virus. The method comprises the following steps of: depending on DNA sequence features of a tomato genome, screening two pairs of CAPs molecular markers which are respectively in close linkage with yellow virus resistant genes Ty-2 and Ty-3, and screening a pair of SCAR molecular markers which are in close linkage with a yellow leaf curl virus resistant gene TY-4; and respectively hybridizing tomato parents containing yellow virus yellow virus resistant genes Ty-2, Ty-3 and Ty-4 and selfing for multiple generations, and identifying an individual plant based on the screened molecular markers, so as to obtain an excellent selfing line which contains Ty-2, Ty-3 and Ty-4 homozygous genes and has stable yellow leaf curl virus resistance. The method disclosed by the invention has advantages of high specificity, good repeatability, low cost and the like, and is capable of accelerating breeding generation, and saving time and labor; and polymerization and purification of three genes can be implemented within a short period, so as to breed a new tomato variety with a stable resistance.

Description

Technical field: [0001] The invention belongs to the field of agricultural breeding, and particularly relates to a tomato breeding method for using molecular markers to polymerize a plurality of tomato yellow leaf curl virus disease resistance genes to select disease-resistant inbred lines. Background technique: [0002] Tomato is an annual or perennial plant of the genus Solanum in the family Solanaceae, native to Peru, Ecuador and other places in South America. Tomato is one of the most important crops with high economic value in the world, and it is also one of the most widely cultivated and highest yield vegetable crops in China. However, nearly 200 diseases have been recorded in cultivated tomatoes. Although producers can use comprehensive strategies including cultivation techniques and pesticides, the introduction of disease resistance genes into modern varieties using traditional breeding methods is still the key to tomato disease resistance. method. [0003] Amo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68A01H1/02
Inventor 卢钢郑积荣陈景丽王洁朱翔飞陈丽妃
Owner ZHEJIANG UNIV