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A method for identifying medicinal materials or derivatives of Cornus officinalis

A technology of dogwood and derivatives, applied in the field of identification of natural medicinal plants, to achieve the effect of strengthening systematization and standardization, high sensitivity, good repeatability and feasibility

Active Publication Date: 2016-01-06
新乡博凯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the influence of non-chromatographic conditions (such as chromatographic column diameter, length, stationary phase grade, carrier particle size, mobile phase flow rate, proportion of components in mixed mobile phase, column temperature, injection volume, detector sensitivity, etc.) There are many limitations in repeatability and feasibility due to large and multiple standard products

Method used

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  • A method for identifying medicinal materials or derivatives of Cornus officinalis
  • A method for identifying medicinal materials or derivatives of Cornus officinalis
  • A method for identifying medicinal materials or derivatives of Cornus officinalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0140] Example 1: IGD carbon NMR fingerprint of the medicinal material Cornus officinalis in Xixia, Henan

[0141] (1) Preparation of characteristic extracts

[0142] Select Cornus officinalis medicinal material from Xixia, Henan Province, and prepare iridoid glycosides characteristic extract of Cornus officinalis medicinal material according to the following method: Weigh 100 g of cornus medicinal material and crush it (over 24 meshes), add 8 times the volume and 20% ethanol twice Reflux extraction at 90°C for 2 times, each extraction for 1 hour, filter, combine the 2 filtrates, and concentrate under reduced pressure; take the concentrated filtrate and use a macroporous adsorption resin (HP-20, diameter Height ratio 12:1) to pack the column, wash the column with 20% ethanol, 50% ethanol and 95% ethanol successively (the volume is 20, 30 and 20 times of the concentrated filtrate, respectively), collect the 50% ethanol eluate, and depressurize Evaporate to dryness to obtain th...

Embodiment 2

[0155] Example 2: IGD Carbon NMR Fingerprint of Danfeng Cornus officinalis in Shaanxi

[0156] (1) Preparation of characteristic extracts

[0157] Select Cornus officinalis from Danfeng, Shaanxi, and prepare the characteristic extract of iridoid glycosides from Cornus officinalis according to the following method: Add 100 g of cornus officinalis that meets the requirements after screening (over 50 mesh), and add the volume 6︰6︰6 times The amount and mass ratio of 25% ethanol was refluxed and extracted at 95°C for 3 times, each extraction was 1.5h, filtered, the filtrates were combined three times, concentrated under reduced pressure; 825 resin was loaded into the column (the ratio of diameter to height is 10:1), and the column was successively washed with 15% ethanol, 45% ethanol and 90% ethanol (the volume of ethanol washed into the column was 25, 40 and 20 times that of the concentrated filtrate, respectively), and the collected The 45% ethanol eluate was evaporated to dryn...

Embodiment 3

[0169] Example 3: Central Plains Zhengxin Cornus officinalis IGD carbon NMR fingerprint

[0170] (1) Preparation of characteristic extracts

[0171] Select the cornus officinalis medicinal material of Zhongyuan Zhengxin, and prepare the characteristic extract of the cornus officinalis iridoid glycosides according to the following method: add 50 g of the cornus officinalis medicinal material (passing 50 meshes) that meets the requirements after screening, and add the volume of 10:10 times, The mass ratio is 20% ethanol, ultrasonic extraction at 60°C for 2 times, each extraction for 1h, filtering, combining the 2 filtrates, and concentrating under reduced pressure; take the concentrated filtrate and pack it into a column with 1 times its weight of HP-20 resin (diameter-to-height ratio is 12:1), successively wash the column with 20% ethanol, 30% ethanol and 95% ethanol (the volume of the ethanol washed into the column is 25, 40 and 20 times that of the concentrated filtrate, resp...

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Abstract

The invention relates to an identification method for a medicinal material (namely Salvia miltiorrhiza Bge.) or derivatives. The method comprises the following steps of: (1) extracting the medicinal material or the derivatives to obtain an active-ingredient-group-containing glycoside feature extract of the Salvia miltiorrhiza Bge.; (2) carrying out IGD C NMR (carbon nuclear magnetic resonance) finger-print detection on the iridoid glycoside feature extract of the Salvia miltiorrhiza Bge. so as to obtain the intensity of the characteristic peaks of a plurality of active ingredients in the iridoid glycoside feature extract of the Salvia miltiorrhiza Bge. according to a finger-print, and detecting the intensity of the characteristic peaks of standard reference substances which correspond to the active ingredients in the same way; (3) detecting the absolute content of the standard reference substances by a quantitative analysis means; and (4) computing the content of each active ingredient and the content of the active ingredient group in the medicinal material or the derivatives according to the specific value and the absolute content of the intensity of the characteristic peaks. The method can be used for reflecting the variety of the iridoid glycoside compounds in the Salvia miltiorrhiza Bge. and the proportion of the compounds, so that the aim for identifying the variety and the quality of the medicinal material can be achieved.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identifying medicinal materials or derivatives of cornus officinalis. Background technique [0002] Cornus officinalis (SalviamiltiorrhizaBge.) is the dried and mature pulp of Cornus officinalis Sieb. et Zucc., a famous Chinese medicinal material in my country. Cornus officinalis belongs to the liver and kidney meridian. It has mild medicinal properties, sour and astringent taste, and has the effect of nourishing the liver and kidney, and reducing astringency and solidification [National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China, 2010 Edition Part One. China Medical Science and Technology Press, 26]. Its active ingredients are mainly iridoid glycosides, which have the functions of lowering blood sugar, resisting hemorrhagic shock, inhibiting platelet aggregation, regulating immunity, antibacterial an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08G01N30/02
Inventor 张海艳赵天增董建军李倩魏悦范毅常霞郭唯王伟李自红
Owner 新乡博凯生物技术有限公司
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