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A method for identifying medicinal materials or derivatives of honeysuckle

A technology of honeysuckle and its derivatives, applied in the field of identification of natural medicinal plants

Active Publication Date: 2016-04-13
河南省科高植物天然产物开发工程技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no relevant reports and technical disclosures using IGD carbon NMR coupled fingerprint technology to identify honeysuckle medicinal materials or derivatives

Method used

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  • A method for identifying medicinal materials or derivatives of honeysuckle
  • A method for identifying medicinal materials or derivatives of honeysuckle
  • A method for identifying medicinal materials or derivatives of honeysuckle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0196] Example 1: Commercially available honeysuckle medicinal material A (Zhongyuan Zhengxin) organic acid and iridoid glycosides characteristic extract IGD C-NMR coupled fingerprint

[0197] (1) Preparation of characteristic extracts

[0198] Weigh 25 grams of commercially available honeysuckle medicinal material A after crushing (passing through a 65 mesh sieve), add 6, 6, and 10 times the volume (150 mL, 150 mL, 250 mL), 20% (volume ratio) of ethanol, and reflux extraction at 90°C 3 times, extract for 1 hour each time, filter and combine the filtrates, concentrate under reduced pressure until there is no alcohol smell, about 500mL, adjust pH=1 with concentrated hydrochloric acid; take the filtrate after acid adjustment, use a macroporous Adsorptive resin (model: HP-20, diameter-to-height ratio 12:1) was packed into a column, washed with water-ethanol system, and 30% of the eluted part was collected to obtain the characteristic extract of honeysuckle A organic acid and irid...

Embodiment 2

[0211] Example 2: Commercially available honeysuckle medicinal material B (Zhongyuan Zhengxin) organic acid and iridoid glycosides characteristic extract IGD C-NMR coupled fingerprint

[0212] (1) Preparation of characteristic extracts

[0213] Weigh 25g of commercially available honeysuckle B (Zhongyuan Zhengxin) decoction pieces after being crushed (passed through a 24 mesh sieve), add 10 or 10 times the volume (250mL, 250mL), 25% (volume ratio) of ethanol, and ultrasonically extract at 50°C 2 times, extracting for 2 hours each time, after filtering, combine the filtrates, concentrate under reduced pressure until there is no alcohol smell, about 500mL, adjust the pH=2 with concentrated hydrochloric acid; take the filtrate after acid adjustment; take the concentrated filtrate, and use its weight as 1 times the amount of macroporous adsorption resin (model: D-101, diameter-to-height ratio 12:1) was packed into a column, washed with water-ethanol system, and 35% of the eluted fra...

Embodiment 3

[0225] Example 3: Commercially available honeysuckle extract IGD carbon nuclear magnetic resonance coupled fingerprint

[0226] (1) Feature extraction selection

[0227] The commercially available chlorogenic acid 20% honeysuckle extract (Hunan Changsha Shanghe Biotechnology Co., Ltd.) was directly selected as the characteristic extract.

[0228] (2) Feature extraction IGD carbon NMR fingerprint detection

[0229] Take 30mg of chlorogenic acid 20% honeysuckle extract, dissolve in 0.5mL deuterated DMSO-d 6 In the process, the IGD carbon-1-1C-1C fingerprint is detected, and the 20% honeysuckle extract IGD-11 carbon-13-1 of chlorogenic acid is obtained.

[0230] (3) IGD carbon NMR fingerprint of chlorogenic acid 20% honeysuckle extract

[0231] 1) IGD carbon NMR fingerprint identification

[0232] In the IGD carbon NMR fingerprint of chlorogenic acid 20% honeysuckle extract, the characteristic signals of organic acid compounds of honeysuckle are clearly displayed. Organic ac...

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Abstract

The invention relates to a method for identifying a lonicera japonica medicinal material and derivatives of the lonicera japonica medicinal material. The method comprises the following steps: (1) extracting the lonicera japonica medicinal material or the derivatives of the lonicera japonica medicinal material, so as to obtain a lonicera japonica organic acid or / and iridoid glycoside characteristic extract containing an active component group; carrying out IGD (Integrated Graphics Driver) nuclear magnetic resonance spectroscopy fingerprint spectrum detection on the characteristic extract so as to obtain peak intensities of characteristic peaks of a plurality of active components in the characteristic extract according to a fingerprint spectrum; measuring the peak intensity of the characteristic peak of a standard reference corresponding to each active component in the same manner so as to obtain the absolute content of the standard reference by adopting a quantitative analysis means; calculating the content of each active component and the content of the active component group in the lonicera japonica medicinal material and the derivatives of the lonicera japonica medicinal material by using a ratio of the peak intensities of the characteristic peaks and the absolute content of the standard reference. The method provided by the invention can be used for reflecting the lonicera japonica organic acid or / and iridoid glycoside compounds contained in the lonicera japonica and a ratio of the lonicera japonica organic acid compounds to the iridoid glycoside compounds, so that the purpose of identifying the variety and the quality of the lonicera japonica medicinal material can be realized.

Description

technical field [0001] The invention belongs to the field of identification of natural medicinal plants, and in particular relates to a method for identifying medicinal materials or derivatives of honeysuckle. Background technique [0002] Honeysuckle (Loniceraejaponicaeflos) is the dry flower bud or the flower with the first bloom of Lonicera japonica Thunb., which is a famous Chinese medicinal material in my country. Honeysuckle belongs to the lung, heart, and stomach meridians. It is cold in nature and sweet in taste, and has the effects of clearing heat, detoxifying, and evacuating wind-heat [National Pharmacopoeia Committee. Pharmacopoeia of the People's Republic of China 2010 Edition Part One. China Medical Science and Technology Press, 205]. Its active ingredients are mainly organic acids, iridoid glycosides and flavonoids, which have the functions of antibacterial and antiviral, antipyretic and anti-inflammatory, liver protection and gallbladder, blood fat lowering, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N24/08G01N30/02
Inventor 张海艳赵天增李坤威张剑董建军陈玲于立芹范毅郭唯李晓
Owner 河南省科高植物天然产物开发工程技术有限公司