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Preparation method for positive serum by porcine reproductive and respiratory syndrome virus

A technology for respiratory syndrome and positive serum, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as the preparation method of porcine reproductive and respiratory syndrome virus positive serum that has not yet been seen, and achieve the goal of reducing foreign virus contamination Risk, the effect of reducing economic costs

Active Publication Date: 2015-01-21
兆丰华生物科技(南京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The patent application number CN201110356735.4 discloses the preparation of positive serum for Newcastle disease hemagglutination inhibition test, the application number CN201010237919.4 discloses the preparation of swine fever positive serum, and the application number CN201010532340.0 discloses the preparation of avian influenza virus H9 subtype positive serum. Preparation, there is no report on the preparation method of porcine reproductive and respiratory syndrome virus positive serum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0017] A preparation method for porcine reproductive and respiratory syndrome virus positive serum, which comprises the following steps:

[0018] 1) Rabbit selection: select rabbits that meet the conditions: adult male rabbits with a weight of 2.0-3.0Kg that meet the first-class animal standards stipulated by the state, and do not contain porcine reproductive and respiratory syndrome virus and its antibodies, swine fever virus and its Antibodies, porcine circovirus type Ⅱ and its antibodies, porcine pseudorabies virus and its antibodies;

[0019] 2) Immunization: immunize each rabbit 3-5 times respectively:

[0020] The first immunization: subcutaneously or intramuscularly inject 1-2.5 parts of porcine reproductive and respiratory syndrome virus live vaccine to each rabbit;

[0021] Multiple immunizations in the middle: After the first immunization, each rabbit is injected subcutaneously or intramuscularly with live porcine reproductive and respiratory syndrome virus vaccine ...

Embodiment 1

[0027] 1) Rabbit selection: select rabbits that meet the conditions: adult male rabbits with a weight of 2.0-3.0Kg that meet the first-class animal standards stipulated by the state, and do not contain porcine reproductive and respiratory syndrome virus and its antibodies, swine fever virus and its Antibodies, porcine circovirus type Ⅱ and its antibodies, porcine pseudorabies virus and its antibodies;

[0028] 2) Immunization: each rabbit was immunized three times:

[0029] The first immunization: subcutaneously or intramuscularly inject 1 dose of porcine reproductive and respiratory syndrome virus live vaccine CH-1R strain to each rabbit;

[0030] The second immunization: 7 days after the first immunization, subcutaneously or intramuscularly inject 2 portions of live porcine reproductive and respiratory syndrome virus vaccine CH-1R strain to each rabbit;

[0031] The last immunization: 7 days after the second immunization, each rabbit was injected intravenously with 0.5mL li...

Embodiment 2

[0035] 1) Rabbit selection: select rabbits that meet the conditions: adult male rabbits with a weight of 2.0-3.0Kg that meet the first-class animal standards stipulated by the state, and do not contain porcine reproductive and respiratory syndrome virus and its antibodies, swine fever virus and its Antibodies, porcine circovirus type Ⅱ and its antibodies, porcine pseudorabies virus and its antibodies;

[0036] 2) Immunization: each rabbit was immunized 4 times:

[0037] The first immunization: subcutaneously or intramuscularly inject 2 portions of porcine reproductive and respiratory syndrome virus live vaccine CH-1R strain to each rabbit;

[0038] The second immunization: 8 days after the first immunization, inject 4 portions of porcine reproductive and respiratory syndrome virus live vaccine CH-1R strain to each rabbit subcutaneously or intramuscularly;

[0039] The third immunization: 8 days after the second immunization, each rabbit was subcutaneously or intramuscularly i...

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Abstract

The invention relates to a preparation method for positive serum by porcine reproductive and respiratory syndrome virus. The preparation method comprises the following steps of: selecting a rabbit, immunizing and vaccinating the rabbit and collecting the rabbit blood serum. According to the preparation method disclosed by the invention, the positive serum is prepared by porcine reproductive and respiratory syndrome virus, and immune antibody-positive serum is obtained by stimulating the porcine reproductive and respiratory syndrome virus through the rabbit body to generate immune response, so that homologous other exogenous virus pollution risks due to homologous animals during the process of preparing the positive serum by a pig are reduced, and a novel scheme is provided for the preparation of the positive serum. Besides, the rabbit, as an experimental animal, is compared with the pig, so that the economic cost is reduced.

Description

technical field [0001] The invention relates to the field of veterinary medicine and biology, in particular to a method for preparing positive serum of porcine reproductive and respiratory syndrome virus. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), commonly known as "pig blue ear disease", is a new RNA virus infectious disease that causes reproductive disorders in sows, respiratory symptoms and high mortality in newborn piglets. The pathogenic porcine reproductive and respiratory syndrome virus was first discovered in the United States in 1987. In 1991, Dutchman Wensvoot et al. first isolated the virus from sick piglets and sows. It was called Lelystad virus (LV) at that time. Subsequently, the virus was also isolated from Germany, the United States, and the United Kingdom. At present, the disease has spread throughout North America and Europe, spreading globally, and the Asia-Pacific region is also spreading. There are outbreaks in Ja...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531
Inventor 陈晟生詹先强陈景容
Owner 兆丰华生物科技(南京)有限公司