A method for preparing infectious bursal virus live vaccine by using subcultured chicken embryo fibroblasts

A fibroblast and infectious technology, applied in the field of biological vaccines, can solve the problems of large batch-to-batch difference in product quality, high cost, and infection of chicken-derived potential diseases, and achieve production cost reduction, extended time, and uniform product quality Effect

Active Publication Date: 2014-10-01
哈药集团生物疫苗有限公司
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AI Technical Summary

Problems solved by technology

At present, the vast majority of commercially available bursal vaccines are chicken embryo tissue vaccines. Compared with cell vaccines, they have the disadvantages of high cost, large batch-to-batch difference in product quality, and easy infection of chicken-derived potential diseases.

Method used

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  • A method for preparing infectious bursal virus live vaccine by using subcultured chicken embryo fibroblasts

Examples

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Embodiment 1

[0053] Example 1 Production of IBDV H11 Live Vaccine Using Passage Chicken Embryo Fibroblasts

[0054] (1) Preparation of poisonous seeds for production

[0055] ① Breeding of poisonous species

[0056] The virus seed H11 strain (preservation number is CGMCC NO.6910) was prepared with sterilized physiological saline for 10 3 ~10 4After one-fold dilution, inoculate a monolayer of chicken embryo fibroblasts; discard the cell culture medium, inoculate according to 1 / 10 of the volume of the culture medium, absorb at 37°C for 1 hour, add the culture medium, and add 10% of 1% volume to it Reduced glutathione solution; 37°C, 5% CO 2 Under certain conditions, culture for 3 to 4 days, and harvest the virus liquid when 80% of the cells have specific lesions; freeze and thaw three times in a -20°C freezer, harvest in a sterilized container, and store at -20°C;

[0057] ② Identification of virus species

[0058] Comply with the production seed standard;

[0059] ③Preservation of poi...

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Abstract

The invention relates to a method for preparing an infectious bursal disease virus live vaccine by utilizing a passage chicken embryo fibroblast. By utilizing the method, the IBDV (infectious bursal disease virus) live vaccine is prepared by steps of preparing a production virus seed, preparing a vaccine preparing material, preparing an IBDV cell virus solution, inspecting a finished product, preparing a vaccine, performing split charging, freeze-drying, and the like. According to the method, the existing production method is improved in aspects of inoculation material, culture solution improvement, culture environment maintenance, freeze-drying of protective agent components, and the like, and a new passage cell culture method is originally created. In addition, the invention also comprises an application of the IBDV live vaccine prepared by the method in prevention of an infectious bursal disease. The IBDV live vaccine prepared by the method has the characteristics of good heat resistance, high virus titer and good stability and is suitable for large-scale vaccine production.

Description

technical field [0001] The invention relates to a method for preparing infectious bursal virus live vaccine by using subcultured chicken embryo fibroblasts, and belongs to the field of biological vaccines. Background technique [0002] Chicken infectious bursal disease (Infeetious Bursal Disease, IBD) is a highly contagious infectious disease caused by infectious bursal virus (Infeetious Bursal Disease Virus, IBDV), which is currently the main infection that endangers the poultry industry in the world. One of the diseases, mainly infringes on the bursa organs of chicks and young chickens. The harm of the disease not only directly causes the death of chickens, increases the elimination rate, affects the weight gain, but more importantly, destroys the B lymphocytes in the bursa, resulting in Immunosuppression makes sick chickens more susceptible to other diseases, and the immune response to vaccination is reduced or lost. [0003] Because IBD is relatively stable in the exter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61K9/19A61P31/14C12N7/00
Inventor 刘洪斌张杨杨万秋丁国杰李东伟杨朋欣焦利红孙心李敏张明艳
Owner 哈药集团生物疫苗有限公司
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