Veterinary bovine ephemeral fever inactivated vaccine and large-scale production method thereof

Abstract

The invention discloses a veterinary bovine ephemeral fever inactivated vaccine and a large-scale production method thereof, and belongs to the technical field of vaccine production. The method provided by the invention comprises the steps of performing cell culture by utilizing a suspension culture process, performing virus suspension culture, performing virus antigen solution clarification, performing concentration and purification, performing inactivation and performing emulsification vaccine preparation, and the method can realize mass proliferation of the bovine ephemeral fever virus, improves the content and yield of the virus antigen, and has a high automation degree; The synergistic effect of all the steps can jointly improve the quality and stability of the vaccine, and the technical problems that the yield of the bovine ephemeral fever virus produced by cell culture through a traditional spinner bottle or microcarrier process is low, the process is tedious, the difference between virus antigen batches is large, the vaccine efficacy is low, the vaccine validity period is short and the like are solved.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation in biomedicine, and in particular relates to a veterinary bovine epidemic fever inactivated vaccine and a large-scale production method thereof. Background technique [0002] Bovine ephemeral fever (BEF) is an acute, non-contact infectious disease of cattle, dairy cattle, beef cattle and buffalo caused by bovine ephemeral fever virus. The disease is characterized by sudden high fever, shortness of breath, lacrimation, and severe catarrhal inflammation and dyskinesia of the digestive organs. Most of the sick cattle infected with the disease can return to normal after 2-3 days, so it is also called three-day fever or temporary fever. The disease is rapid and violent, but mostly benign. However, the disease can cause large herds of cattle to fall ill and significantly reduce the milk production of dairy cows. [0003] The bovine epidemic fever vaccine is one of the most important ways t...

AI Technical Summary

Problems solved by technology

The preparation of these two viral antigens requires adherent cell culture, and the cells need to be digested and divided into bottles during the large-scale production of vaccines, which has a high risk of contamination; moreover, these operations need to be done manually, and a large number of manual operations make labor costs high

In addition, the number of adherent cells is small, and there are technical defects such as low toxin production efficiency, low vaccine efficacy, large immune doses required to achieve the same immune effect, and large batch-to-batch differences.

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