Novel mushroom residue and arbuscular mycorrhizal fungi (AMF) culture medium, and preparation method and application thereof

A technology for cultivation substrates and cultivation substrates, applied in the directions of botanical equipment and methods, applications, fertilizer mixtures, etc., can solve the problems of occupying land, increasing medium waste, polluting the environment, etc., to promote vegetable growth, improve circular economic benefits, The effect of protecting the environment

Inactive Publication Date: 2014-12-17
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a new type of cultivation substrate of fungus chaff + AMF and its preparation method and application, which can increase the existing methods for solving the problems of the accumulation of culture medium waste in the production of edible fungi, polluting the environment and occupying land.

Method used

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  • Novel mushroom residue and arbuscular mycorrhizal fungi (AMF) culture medium, and preparation method and application thereof
  • Novel mushroom residue and arbuscular mycorrhizal fungi (AMF) culture medium, and preparation method and application thereof
  • Novel mushroom residue and arbuscular mycorrhizal fungi (AMF) culture medium, and preparation method and application thereof

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Embodiment 1

[0025] The present embodiment utilizes the method for recycling and reusing the novel cultivation substrate of the bacterium chaff+AMF comprising the following steps:

[0026] 1. Preparation of fungus bran + AMF new cultivation medium

[0027] Fine river sand and clay that have passed through a 2mm sieve without any impurities are selected, mixed according to fine river sand:clay=5:2~1 (volume ratio), and used as AMF culture substrate after moist heat sterilization. Add 500-1000 spores of Glomus intaradices (separated and preserved in Qingdao Agricultural University, preservation number: Gi95) to the above 2-3L culture medium, sow tobacco and clover, and cultivate in the greenhouse: natural light conditions; day and night temperatures are 25 ~30℃ and 12~15℃; water as needed; apply 20%~30% Hoagland nutrient solution every two weeks. Others are under conventional management. Harvest the substrate and root system after 3 to 4 months of cultivation. Adjust the number of inoculu...

Embodiment 2

[0044] The present embodiment utilizes the method for recycling and reusing the novel cultivation substrate of the bacterium chaff+AMF comprising the following steps:

[0045] 1. Fine river sand and clay that have passed through a 2mm sieve without any impurities are selected, mixed according to fine river sand:clay=5:2~1 (volume ratio), and used as AMF culture substrate after moist heat sterilization. Add 500-1000 spores of Glomus intaradices (isolated and preserved by Qingdao Agricultural University, preservation number: Gi95) to the above-mentioned 2-3L culture medium and sow tobacco and clover, and cultivate them in a greenhouse under natural light conditions; the day and night temperature is 25-25 30-12~15℃; water as needed; apply 20%~30% Hoagland nutrient solution every two weeks. Others are under conventional management. Harvest the substrate and root system after 3 to 4 months of cultivation. Adjust the inoculum IPU quantity to 15000IPU L -1 spare;

[0046] The ch...

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Abstract

The invention provides a novel mushroom residue and arbuscular mycorrhizal fungi (AMF) culture medium, and a preparation method and application thereof. The preparation method mainly comprises the steps of: inoculating arbuscular mycorrhizal fungi (AMF) with mushroom residues so as to prepare the mushroom residue and AMF culture medium; planting tomato, strawberry or onion seedlings on the medium so as to grow and culture vegetables; after the vegetable growing seasons are finished, reusing the medium which cultures vegetables as an AMF inoculation agent and a soil organic additive, and applying the medium to protect soil. The organic matter, AMF inoculation material and mineral contents in the soil can be increased, soil physical and chemical properties are improved, and soil fertility is increased; plant growth is stimulated, yield is increased, and quality is improved; and the purposes of efficient mushroom residue recycling, environmental protection, agricultural sustainable production promotion and circular economy are realized.

Description

technical field [0001] The invention belongs to the technical field of resource and environment protection, and in particular relates to a novel culture substrate of fungus chaff+AMF and its preparation method and application. Background technique [0002] Edible fungus is a general term for a class of edible large fungi with nutritional value, medicinal value and economic value. The large-scale production of edible fungi not only meets people's large demand for food, but also drives economic development. At present, the development of edible fungi industry in various countries in the world is growing day by day. However, with the continuous expansion of the production scale of edible fungi, the amount of solid medium discarded during the production of edible fungi, i.e. fungus chaff, is also increasing. Investigations show that about 3.25kg of fungus chaff is produced for every 1kg of edible fungus produced. According to statistics, the amount of fungus bran produced in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C05G3/04A01G1/04C05G3/80
Inventor 刘润进李莉
Owner QINGDAO AGRI UNIV
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