Novel mushroom residue and arbuscular mycorrhizal fungi (AMF) culture medium, and preparation method and application thereof
A technology for cultivation substrates and cultivation substrates, applied in the directions of botanical equipment and methods, applications, fertilizer mixtures, etc., can solve the problems of occupying land, increasing medium waste, polluting the environment, etc., to promote vegetable growth, improve circular economic benefits, The effect of protecting the environment
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Embodiment 1
[0025] The present embodiment utilizes the method for recycling and reusing the novel cultivation substrate of the bacterium chaff+AMF comprising the following steps:
[0026] 1. Preparation of fungus bran + AMF new cultivation medium
[0027] Fine river sand and clay that have passed through a 2mm sieve without any impurities are selected, mixed according to fine river sand:clay=5:2~1 (volume ratio), and used as AMF culture substrate after moist heat sterilization. Add 500-1000 spores of Glomus intaradices (separated and preserved in Qingdao Agricultural University, preservation number: Gi95) to the above 2-3L culture medium, sow tobacco and clover, and cultivate in the greenhouse: natural light conditions; day and night temperatures are 25 ~30℃ and 12~15℃; water as needed; apply 20%~30% Hoagland nutrient solution every two weeks. Others are under conventional management. Harvest the substrate and root system after 3 to 4 months of cultivation. Adjust the number of inoculu...
Embodiment 2
[0044] The present embodiment utilizes the method for recycling and reusing the novel cultivation substrate of the bacterium chaff+AMF comprising the following steps:
[0045] 1. Fine river sand and clay that have passed through a 2mm sieve without any impurities are selected, mixed according to fine river sand:clay=5:2~1 (volume ratio), and used as AMF culture substrate after moist heat sterilization. Add 500-1000 spores of Glomus intaradices (isolated and preserved by Qingdao Agricultural University, preservation number: Gi95) to the above-mentioned 2-3L culture medium and sow tobacco and clover, and cultivate them in a greenhouse under natural light conditions; the day and night temperature is 25-25 30-12~15℃; water as needed; apply 20%~30% Hoagland nutrient solution every two weeks. Others are under conventional management. Harvest the substrate and root system after 3 to 4 months of cultivation. Adjust the inoculum IPU quantity to 15000IPU L -1 spare;
[0046] The ch...
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