Method of producing pancreatic hormone-producing cells

一种细胞、胰腺的技术，应用在制备胰腺激素产生细胞领域，能够解决胰岛素产生效率低等问题
CN103154240AInactive Publication Date: 2013-06-12TAKEDA PHARMA CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TAKEDA PHARMA CO LTD
Publication Date
2013-06-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The purpose of the present invention is to provide a method of producing pancreatic hormone-producing cells that have a form more similar to that of normally developed pancreatic cells from stem cells. The method of producing pancreatic hormone-producing cells is characterized by subjecting stem cells to the following steps (1)-(6): (1) a step for culturing stem cells in a culture medium that includes a Rho kinase inhibitor; (2) a step for culturing the cells obtained in step (1) in a culture medium including a GSK3 inhibitor; (3) a step for culturing the cells obtained in step (2) in a culture medium including a GSK3 inhibitor and an agent that activates activin receptor-like kinases 4 and 7; (4) a step for forming a cell aggregate of the cells obtained in step (3) and culturing said cell aggregate in a floating state within a culture medium; (5) a step for culturing the cells obtained in step (4) in a culture medium including a retinoic acid receptor agonist, an AMP-activated protein kinase and / or an activin receptor-like kinase 2, 3 and 6 inhibitor, an activin receptor-like kinase 4, 5 and 7 inhibitor and a cell growth factor; and (6) a step for culturing the cells obtained in step (5) in a culture medium.
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Description

technical field

[0001] The present invention relates to a method for producing pancreatic hormone-producing cells, and a medicament comprising the pancreatic hormone-producing cells obtained by the production method. Background of the invention

[0002] The pancreas has endocrine glands (endocrine cells) and exocrine glands (exocrine cells), and is an organ that plays an important role through two types of cells. Exocrine cells mainly function to secrete digestive enzymes such as pancreatic lipase, trypsin, elastase, and pancreatic amylase, for example.

[0003] Endocrine cells play a role in secreting pancreatic hormones. It is known that pancreatic α cells secrete glucagon, pancreatic β cells secrete insulin, pancreatic δ cells secrete somatostatin, and PP cells secrete pancreatic polypeptide (sometimes in this specification abbreviated as PP). Furthermore, in recent years, it has been reported that ghrelin, which is a hormone secreted by the stomach, is also secreted by...

Claims

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