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Production method for fermentation medium of brown mushroom vegetable garden soil

A vegetable garden soil and culture substrate technology, applied in the field of culture substrate for the cultivation of brown mushroom liquid strains, can solve the problems that liquid strains are not suitable for direct inoculation, inconvenient storage and transportation, etc. The effect of a wide range and an increase in the dry weight of mycelia

Inactive Publication Date: 2013-06-19
天津市金三农农业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, liquid strains are not suitable for direct inoculation on large quantities of compost culture materials, because storage and transportation are inconvenient

Method used

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  • Production method for fermentation medium of brown mushroom vegetable garden soil
  • Production method for fermentation medium of brown mushroom vegetable garden soil
  • Production method for fermentation medium of brown mushroom vegetable garden soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Test materials and methods

[0025] Tested strains: Brown mushroom MC441 was quoted from the Fujian Provincial Research and Extension Station of Mushroom Strains and is available for sale.

[0026] Substrate for test culture:

[0027] (1) Incline culture substrate: Peat soil culture substrate (g / L): 600g peat soil, 20g glucose, 100g wheat grains, 1.5g magnesium sulfate, 1g potassium dihydrogen phosphate, 2g peptone, 1000mL water.

[0028] (2) Seed culture medium (g / L): potato 200, glucose 20, peptone 2, potassium dihydrogen phosphate 2, magnesium sulfate 1, VB 1 0.01, water 1000mL, pH natural.

[0029] (3) Matrix basal fermentation culture medium:

[0030] A. PDA culture medium: 200 g potato, 20 g glucose, and 1000 mL water.

[0031] B. Wheat kernel culture medium Ⅰ: 100 g potato, 1.5 g magnesium sulfate heptahydrate, 1 g potassium dihydrogen phosphate, 2 g peptone, 1 000 mL water.

[0032] C. Wheat kernel culture medium II: 200 g wheat kernels, 1.5 g magnes...

Embodiment 2

[0086] (1) Preparation of seed liquid, take 5 pieces of 0.5 cm 2 Fresh slant strains were inoculated in the seed culture medium (50mL / 250mL Erlenmeyer flask), cultured on a shaker at 25°C and 150rpm for 3 days; the slant culture medium: peat soil culture medium (g / L): peat soil 600g, glucose 20g , wheat grain 100g, magnesium sulfate 1.5g, potassium dihydrogen phosphate 1g, peptone 2g, water 1000mL; seed culture medium (g / L): potato 200, glucose 20, peptone 2, potassium dihydrogen phosphate 2, magnesium sulfate 1, VB1 0.01, water 1000mL, pH natural;

[0087] (2) For the preparation of liquid strains, transfer the seed liquid into 50mL with 10% (v / v) inoculation amount, and the fermentation medium is (g / L): vegetable garden soil 700, wheat grain 120, peptone 4, glucose 15, Magnesium sulfate heptahydrate 1.5, potassium dihydrogen phosphate 1: bran 120, glucose 15, peptone 2 in a 250mL triangular flask, cultured at 25°C and 150rpm for 7 days to obtain brown mushroom liquid strain...

Embodiment 3

[0091] (1) Preparation of seed liquid, take 5 pieces of 0.5 cm 2Fresh slant strains were inoculated in the seed culture medium (50mL / 250mL Erlenmeyer flask), cultured on a shaker at 25°C and 150rpm for 3 days; the slant culture medium: peat soil culture medium (g / L): peat soil 600g, glucose 20g , wheat grain 100g, magnesium sulfate 1.5g, potassium dihydrogen phosphate 1g, peptone 2g, water 1000mL; seed culture medium (g / L): potato 200, glucose 20, peptone 2, potassium dihydrogen phosphate 2, magnesium sulfate 1, VB1 0.01, water 1000mL, pH natural;

[0092] (2) For the preparation of liquid strains, transfer the seed liquid into 50mL with 10% (v / v) inoculation amount, and the fermentation medium is (g / L): vegetable garden soil 700, wheat grain 120, peptone 4, glucose 15, Magnesium sulfate heptahydrate 1.5, potassium dihydrogen phosphate 1: bran 120, glucose 15, peptone 2 in a 250mL triangular flask, cultured at 25°C and 150rpm for 7 days to obtain brown mushroom liquid strains...

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Abstract

The invention provides a production method for a culture medium for brown mushroom liquid strains. The production method comprises the specific steps of preparing the culture medium, wherein the fermentation medium of vegetable garden soil comprises 700 (g / L) of the vegetable garden soil, 120 (g / L) of wheat grains, 4 (g / L) of peptone, 15 (g / L) of glucose, 1.5 (g / L) of magnesium sulfate heptahydrate, and 1 (g / L) of monopotassium phosphate; the rotational speed is 100 rpm, the liquid-loading amount is 75 mL / 250 mL, and the inoculum size is 12% (w / v); and in the conditions, the mycelial yield of brown mushrooms is increased to 17.028 g / L from 1.657 g / L. The method provided by the invention meets the needs of people and market on new mushroom strains and novel edible mushroom technologies, and certainly generates great economic benefits and social benefits in case of being widely applied.

Description

technical field [0001] The invention provides a cultivation method related to brown mushrooms, more specifically a culture substrate for cultivating liquid strains of brown mushrooms and a cultivation method thereof. Background technique [0002] brown mushrooms ( Agaricus brunnescens Peck), is a rare species of mushrooms, belonging to Basidiomycotina (Basidiomycotina), Agaritales (Agaritales), mushroom family (Agaricaceae), mushroom genus ( Garicus ). Brown mushrooms, commonly known as brown mushrooms, are Bohemian subspecies. The strain has high yield, excellent mushroom quality, and fresh and tender taste. The mushroom cap is natural brown, the stipe is white, and the meat is dense and firm. All current white Agaricus species are white varieties of the brown mushroom, which means that the brown mushroom is the wild ancestor of the white Agaricus bisporus. Brown mushrooms, also known as white mushrooms and champignons, belong to Basidiomycetes, Agaricaceae, Agaricacea...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01G1/04
Inventor 班立桐黄亮王玉杨丽维孙茜周阳朱枷华
Owner 天津市金三农农业科技开发有限公司
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