Method of rapidly eliminating antibiotic-resistant plasmids in E. coli
A technology of Escherichia coli and antibiotics, applied in the field of microbial genetics, to achieve good results, high plasmid elimination rate, and promote breakdown
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[0047] Escherichia coli SCS110, JM109, and DH5α contain the antibiotic resistance plasmid pK19mobsacB, which belongs to Gram-negative bacteria. Because there is a kanamycin resistance gene on the pK19mobsacB plasmid, Escherichia coli SCS110, JM109, and DH5α containing the pK19mobsacB plasmid can all be used in the Grow on kanamycin-resistant plates.
[0048] A method for obtaining Escherichia coli that eliminates antibiotic resistance plasmids after electric shocking human enterobacteria, the steps are as follows:
[0049] A. Preparation of competent cells:
[0050] (1) Pick the antibiotic-resistant plasmid pk19mobsacB from the LB plate (Schafer A, Tauch A, Jager W, et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in The chromosome of Corynebacterium glutamicum.Gene, 1994, 145 (1): 69-73) Escherichia coli single bacterium colony, such as Escherichia coli SCS110 strain single bacteri...
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