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Method for magnetically and rotatably applying bacteria on culture medium panel

A medium and magnetic spin technology, applied in the field of microbial experiments, to reduce human error, shorten preparation time, and improve coating efficiency.

Inactive Publication Date: 2013-06-26
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This device spreads well, but still requires the media plate to be left open to operate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select a 60mm medium plate, and the stainless steel magnetic stirring bar is a cylinder with a diameter of 3mm, and the length is less than the inner diameter of the medium plate; put the magnetic stirring bar in a heat-resistant container, and after moist heat sterilization, dry it and cool it to room temperature for later use; Set the magnetic stirring bar on the central axis of the medium plate, drop the bacterial suspension on the medium corresponding to the central point of the magnetic stirring bar, and then cover the culture dish cover; place the medium plate on the magnetic stirrer, and magnetically The stirrer drives the magnetic stirring bar to rotate clockwise on the surface of the medium plate for 1 min, and evenly coats the bacterial suspension on the surface of the medium plate; after the coating is completed, take out the magnetic stirring bar for disinfection and cleaning for reuse.

Embodiment 2

[0024] Select a 90mm medium plate, and the magnetic ferromagnetic stirrer coated with polytetrafluoroethylene is a cylinder with a diameter of 4mm, and the length is less than the inner diameter of the medium plate; the magnetic stirrer is placed in a heat-resistant container and sterilized by dry heat Finally, dry, cool to room temperature for later use; set the magnetic stirring bar on the central axis of the medium plate, drop the bacterial suspension on the medium at the position corresponding to the center point of the magnetic stirring bar, and then cover the culture dish; put the medium The plate is placed on a magnetic stirrer, and the magnetic stirrer drives the magnetic stirring bar to rotate counterclockwise on the surface of the medium plate for 2 minutes, and the bacterial suspension is evenly coated on the surface of the medium plate; after the coating is completed, take out the magnetic stirring bar for disinfection and cleaning. reuse.

Embodiment 3

[0026] Choose a 100mm medium plate, and the ordinary ferromagnetic stirring bar coated with polytetrafluoroethylene is a cylinder with a diameter of 6mm, and the length is smaller than the inner diameter of the medium plate; , dried, and cooled to room temperature for later use; set the magnetic stirring bar on the central axis of the medium plate, drop the bacterial suspension on the medium corresponding to the center point of the magnetic stirring bar, and then cover the culture dish; place the medium plate Place it on a magnetic stirrer, and the magnetic stirrer drives the magnetic stirring bar to alternately rotate clockwise and counterclockwise on the surface of the medium plate for 2 minutes, and spread the bacterial suspension evenly on the surface of the medium plate; after the coating is completed, take out the magnetic stirring bar for disinfection Wash and save for reuse.

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Abstract

The invention discloses a method for magnetically and rotatably applying bacteria on a culture medium panel. The method comprises the steps of: heating, sterilizing, drying and cooling a magnetic stirring rod to the room temperature for standby application, wherein the length of the magnetic stirring rod is less than the internal diameter of the culture medium panel; arranging the magnetic stirring rod on a central axis of the culture medium panel, dripping a bacterium suspension liquid onto a culture medium through aiming at a central point position of the magnetic stirring rod, and then, covering a petri dish cover; putting the culture medium panel on a magnetic stirring apparatus, driving the magnetic stirring rod to rotate on the surface of the culture medium panel through the magnetic stirring apparatus, and uniformly applying the bacterium suspension liquid on the surface of the culture medium panel; and after the applying is finished, taking the magnetic stirring rod out, and sterilizing and cleaning the magnetic stirring rod for being repeated use. According to the method, the magnetic stirring rod serves as an application tool, and the bacterium suspension liquid is uniformly applied on the surface of the culture medium panel through utilizing the horizontal rotation of the magnetic stirring rod. The non-contact drive of the magnetic stirring rod is ingeniously utilized to apply bacteria in a closed state, so that the bacteria application efficiency is increased, and the operation errors of operators can be reduced.

Description

technical field [0001] The invention belongs to the technical field of microbial experiments, and in particular relates to a method for magnetically spinning bacteria on a culture medium plate. Background technique [0002] Spreading bacteria on culture medium plates is a commonly used method in microbiological experiments, and is widely used in operations such as bacterial isolation, bacterial counting, drug susceptibility testing, and antagonistic testing. Bacteria are usually coated by hand with a coating rod. Although it is simple, this method is easy to scratch the surface of the medium, it is difficult to coat evenly, and it is prone to problems such as human error, and the operation of the coating rod requires that the plate must be open or semi-open. state, the medium is in contact with the air for a long time, which is easy to cause bacterial contamination. For this reason, a bacteria spreading and inoculating instrument has appeared, which consists of a rotatable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 方敦煌陈学军李永平肖炳光
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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