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Microalgae collection method and application

A technology for microalgae and microalgae cells is applied in the field of collection and application of microalgae, which can solve the problems of large amount of flocculant, high harvesting cost, and speed up, and achieves the effects of low cost, increased light intensity, and accelerated photosynthesis.

Active Publication Date: 2013-07-03
ENN SCI & TECH DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The present invention has utilized above-mentioned abnormal phenomenon, has taken to keep CO 2 Sustained lack or artificially accelerated CO 2 The absence of a process further increases the pH value of the algae liquid, thereby promoting the rapid occurrence of cell self-flocculation, realizing the low-cost harvesting of microalgae without adding additional flocculants, and solving the problems of large flocculant consumption and harvesting costs in the prior art advanced questions

Method used

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  • Microalgae collection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] see figure 2 .

[0053] Seawater chlorella (Chlorella sp.) is in a normal growth state in a plate-type photobioreactor with a volume of 50L. When it grows to the late exponential or plateau stage, the gas that will be introduced at this time will change from a carbon dioxide concentration of 10 volume % of the mixed gas is changed to air, the flow rate of ventilation is 1.5-3.0L / min, the pH value of the microalgae culture solution rises from 7.53 to 9.50 after 3 hours of ventilation, and the pH value rises to 10.40 after 5 hours of ventilation, and the ventilation is stopped. The microalgae cells in the algae liquid to be harvested are self-flocculated and settled for 2 hours, and the concentrated solution after sedimentation is discharged through the discharge valve to obtain the microalgae concentrated solution. The concentration of the microalgae after concentration is the microalgae culture solution 30 times the concentration.

Embodiment 2

[0055] see image 3 .

[0056] Nannochloropsis sp is in a normal growth state in a plate-type photobioreactor with a volume of 50L. When it grows to the post-exponential or plateau stage, the gas will be fed with a carbon dioxide concentration of 10% by volume. The mixed gas of the mixture was changed to nitrogen gas, the flow rate of the ventilation was 1.5-3.0L / min, and the applied light intensity was 400μmol / m 2 s artificial light source or natural light, the pH value of the microalgae culture solution rises from 7.31 to 9.80 after 2 hours of nitrogen gas flow, and rises to 10.40 after 3 hours of nitrogen gas flow, stop the ventilation, and the microalgae cells in the algae liquid are to be harvested Flocculation and sedimentation, let stand for 2 hours, discharge the settled concentrated solution through the discharge valve to obtain the microalgae concentrated solution, the concentration of the concentrated microalgae is 31 times of the concentration of the microalgae cu...

Embodiment 3

[0058] see Figure 4 .

[0059] Seawater chlorella (Chlorella sp.) is in a normal growth state in a plate-type photobioreactor with a volume of 300L. When it grows to the late exponential or plateau stage, the gas will be fed with a carbon dioxide concentration of 10% by volume. The mixed gas of the mixture is changed to air (the concentration of carbon dioxide is 0.4 volume %), and the flow rate of ventilation is 1.5-3.0 L / min. At this time, an artificial light source is added, and the light intensity is 300 μmol / m 2 s, the pH value of the microalgae culture solution rose from 7.31 to 9.50 after 3 hours, and rose to 10.40 after 5 hours, the aeration was stopped, and the microalgae cells in the algae solution to be harvested appeared flocculation, at this time, the flocculated microalgae were input into the air Floatation device, the air flotation device is used to feed microbubbles (the volume of a single microbubble is 700nm ~ 12μm, and the gas component can be air, inert g...

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Abstract

The invention provides a microalgae collection method without adding any flocculating agent. The method comprises the steps of (1) reducing CO2 concentration in an algae solution while culturing the microalgae to make a pH value of the algae culture solution rise, when the pH value rises to 9.50-11.00, the microalgae cells start settling after self-flocculation, a settled algae solution is discharged to obtain a concentrated algae solution, or a supernatant liquid is discharged and discarded to obtain the concentrated algae solution; and (2) introducing micro bubbles when the algae cells start floating up to accelerate floatation of the algae cells and to discharge the algae cells along a guiding device to obtain the concentrated algae solution; or (3) introducing the micro bubbles when the microalgae cells self-flocculate or after the self-flocculation so as to accelerate floatation of the algae cells and to discharge the algae cells along the guiding device to obtain the concentrated algae solution. The invention also provides an application of the microalgae collection method in the culture of the microalgae.

Description

technical field [0001] The invention relates to a method for collecting microalgae and its application. Specifically, the present invention provides a method for collecting microalgae without adding flocculants and its application in microalgae cultivation. Background technique [0002] Microalgae are a class of individual tiny algae, most of which are aquatic eukaryotes, which are single-celled or composed of several cells. Microalgae are rich in nutrients, rich in protein, vitamins and polyunsaturated fatty acids, etc., and have fast growth and high yield per unit area. Based on these characteristics, microalgae have a very wide range of applications in industrial fields such as bait, health food, pharmaceuticals and biofuels. However, due to the small size of microalgae (the diameter of microalgae is generally 1-20 μm), and the concentration in the culture solution is very low (usually <1g / L), it is very difficult to harvest it. For industrial large-scale microalgae...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 吴洪张晋阳杨丽娜纪委刘敏胜朱振旗陈彦平
Owner ENN SCI & TECH DEV
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