Method and fermentation culturing medium used for producing natamycin
A technology for producing natamycin and fermentation medium, which is applied in the field of bioengineering and can solve the problems of slow progress in industrial production, insufficient depth and system, and low level of natamycin fermentation
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Embodiment 1
[0033] 1. Preparation of seeds: inoculate Streptomyces natalensis ATCC 27448 on a solid slant medium, culture at 28°C for 8 days after activation; then make a spore suspension with sterile normal saline, press 4% (V / V) Inoculated into a 150ml shake flask containing 30ml of liquid seed culture medium, and cultivated at 28°C and 150r / min for 2 days.
[0034] The solid slant medium is: 15 g glucose, 5 g peptone, 3 g beef extract, 100 mL fresh wort, agar 17, deionized water to a constant volume of 1000 mL, and pH 7.0 after sterilization.
[0035] The liquid seed culture medium is 15 g glucose, 2 g soy peptone, 2 g corn steep liquor, 10 sodium chloride, constant volume of water to 1000 mL, and pH 7.0 after sterilization.
[0036] 2. Preparation of biological elicitor: inoculate Streptomyces griseus AS 4.1095 in a solid slant medium for activation and culture for 4 days, then make a spore suspension with sterile normal saline, and inoculate it with 2% (V / V) inoculum In a 250ml shake flask...
Embodiment 2
[0044] 1. Preparation of seeds: inoculate Streptomyces natalensis ATCC 27448 on a solid slant medium, culture at 26°C for 6 days after activation; then use sterile normal saline to make a spore suspension, press 4% (V / V) Inoculated into a 150ml shake flask containing 30ml of liquid seed culture medium at a ratio of, and cultivated for 2 days at 26°C and 120r / min.
[0045] The solid slant medium is: glucose 15g, peptone 1g, beef extract 1g, fresh wort 200mL, agar 15, deionized water to a constant volume of 1000mL, pH 6.8 after sterilization.
[0046] The liquid seed culture medium is: 10 g glucose, 5 g soy peptone, 1 g corn syrup, 5 g sodium chloride, constant volume of water to 1000 mL, and pH 6.8 after sterilization.
[0047] 2. Preparation of biological elicitor: inoculate Streptomyces griseus AS 4.1095 in a solid slant medium for activation and culture for 4 days, then make a spore suspension with sterile normal saline, and inoculate it with 2% (V / V) inoculum In a 250ml shake fla...
Embodiment 3
[0055] 1. Preparation of seeds: inoculate Streptomyces natalensis ATCC 27448 on a solid slant medium, culture at 30°C for 10 days and activate; then use sterile normal saline to make a spore suspension, press 4% (V / V) Inoculated into a 150ml shake flask containing 30ml of liquid seed culture medium, and cultivated at 30°C and 160r / min for 5 days.
[0056] The solid slant culture medium is: glucose 5g, peptone 10g, beef extract 5g, fresh wort 50ml, agar 20g, deionized water to 1000ml, pH 7.2 after sterilization.
[0057] The liquid seed culture medium is 20 g glucose, 1 g soy peptone, 5 g corn syrup, 15 g sodium chloride, constant volume of water to 1000 mL, and pH 7.2 after sterilization.
[0058] 2. Preparation of biological elicitor: inoculate Streptomyces griseus AS 4.1095 in a solid slant medium for activation and culture for 4 days, then make a spore suspension with sterile normal saline, and inoculate it with 2% (V / V) inoculum In a 250ml shake flask containing 50ml of liquid c...
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