Forest cultivation method of morchella crassipes
A technology for understory cultivation and morels, applied in the field of understory cultivation of morels thick-legs, can solve the problem of low yield of natural morels thick-legs, achieve significant economic and ecological benefits, increase yields, and solve yield problems low effect
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Embodiment 1
[0030] Embodiment 1 Morchella roughlegs fruit body collection and mycelia separation, purification and medium used
[0031] Fresh, robust 8-9 mature fruiting bodies are taken from fresh and robust morels. Be careful not to collect fruiting bodies in rainy days, so as not to increase the chance of contamination during transportation. Collected fruiting bodies were sorted, photographed, and dated prior to separation.
[0032] The collected fruiting bodies were placed in air-permeable boxes and immediately transported back to the laboratory for isolation. The morel hyphae were isolated by tissue separation and spore separation, and then purified and cultivated.
[0033] The medium used for separation and purification consists of the following components in terms of parts by mass: 20 parts of potato, 2 parts of glucose, 3 parts of humus from the field of morel mushrooms, 2 parts of agar, and 100 parts of water.
Embodiment 2
[0034] Embodiment 2 isolates and purifies the culture condition of morel leg mycelium
[0035] Inoculate the mycelia into a petri dish containing the culture medium. After the inoculation, label it, indicate the name, serial number and date of the strain, and put it in a constant temperature and humidity incubator for cultivation. The cultivation conditions are 24°C and 70°C. %, no light.
Embodiment 3
[0036] The cultivation of embodiment 3 hickory chick thick leg solid cultivation bacterial classification
[0037] The mycelium of the morel leg morel is obtained by separating and purifying the fruit body of the wild morel leg. After the separation and purification of the mycelia, the mycelia which are strong, dense, vigorously growing and evenly distributed are selected for cultivation of solid culture strains.
[0038] The bagged compost for cultivating Morchella roughleg solid culture strains consists of the following components in parts by mass: 78 parts of miscellaneous sawdust, 15 parts of wheat bran, 3 parts of corn flour, 2 parts of sawdust flakes, and 1 part of sucrose part, 1 part of quicklime, and the water content of the compost is 55%-58%.
[0039] According to the formula of compost, mix sawdust, wheat bran, corn flour and sawdust flakes evenly, then pour the melted sucrose, lime and clear water into the mixture, stir evenly, the water content of compost is 55%-...
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