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Application of fructus akebiae extract in preparation of drug for treating primary hepatic carcinoma

A technology of primary liver cancer and predictor, which is applied to the application of the extract of predictor in the treatment of primary liver cancer drugs, and the application field of pharmaceutical preparations, which can solve the problems that have not yet been reported on the extract of predictor

Inactive Publication Date: 2013-07-10
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the literature search, so far there is no report on the use of the extract of oracle seed for inhibiting the malignant proliferation of primary liver cancer cells and its direct related diseases

Method used

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  • Application of fructus akebiae extract in preparation of drug for treating primary hepatic carcinoma
  • Application of fructus akebiae extract in preparation of drug for treating primary hepatic carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: prepare the ethanol extract of each single herbal medicine

[0017] Take 35g each of the four traditional Chinese medicine decoction pieces of Bupleurum, Prediction, Salvia, and peach kernel, dry in an oven at 60°C for 24 hours, and then pulverize.

[0018] Add 350 mL of ethanol aqueous solution with a volume fraction of 75% to the crushed medicinal materials, soak for 2 hours at room temperature, heat to reflux in a water bath, and extract for 2 hours at each reflux, a total of 2 times for reflux extraction; collect the ethanol extract, filter, Concentrate to 25mL respectively, adjust the pH to 7.0 with alkali, then add double distilled water to make up to 35mL (1g crude drug / mL).

[0019] Cell culture conditions Suction filtration sterilization, that is.

Embodiment 2

[0020] Example 2: Experiment of the inhibitory effect of the ethanol extracts of the above-mentioned single herbs on the proliferation of primary liver cancer cells

[0021] Spread SMMC7721 liver cancer cells on a 96-well plate;

[0022] After the cells adhered to the wall overnight, the ethanol extracts of each single drug were administered at a concentration of 5.0 mg / mL; and the cytotoxic antibiotic G418 at a concentration of 0.6 mg / mL was used as a positive control.

[0023] After 24, 48, and 72 hours of administration, the proliferation and growth rate of SMMC7721 liver cancer cells were detected by MTT method, and the dose-effect curve was drawn.

[0024] figure 1 It is a comparison chart of the inhibitory effect of each drug group on the malignant proliferation of SMMC7721 liver cancer cells, in which: a is the normal cell group, b is the G418 group with a drug concentration of 0.6 mg / mL, and c is the ethanol extraction of Bupleurum radix with a drug concentration of 5...

Embodiment 3

[0026] Example 3: Experiment of the inhibitory effect of different doses of the extract of the predictor on the proliferation of primary liver cancer cells

[0027] Spread SMMC7721 liver cancer cells on a 96-well plate;

[0028] After the overnight cell attachment, the extracts of 5 dosages of 1.25mg / mL, 2.5mg / mL, 5.0mg / mL, 10.0mg / mL, and 20.0mg / mL were administered with the final concentration respectively; Cytotoxic antibiotic G418 with a concentration of 0.6 mg / mL was used as a positive control.

[0029] After 24, 48, and 72 hours of administration, the proliferation and growth rate of SMMC7721 liver cancer cells were detected by MTT method, and the dose-effect curve was drawn.

[0030] figure 2 It is a comparison chart of the inhibitory effect of different doses of predictor extracts on the malignant proliferation of SMMC7721 liver cancer cells, where: a is the normal cell group, b is the G418 group with a drug concentration of 0.6 mg / mL, and c is a drug concentration o...

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Abstract

The invention discloses application of a fructus akebiae extract in preparation of a drug for treating primary hepatic carcinoma. The application is that the fructus akebiae extract is taken as an active ingredient to prepare a drug preparation for treating the primary hepatic carcinoma. A cancer cell growth and proliferation MTT (methyl thiazolyl tetrazolium) detection method is adopted by the invention, and the experiment demonstrates that the fructus akebiae extract has the significant effect of restraining malignant cell proliferation of the primary hepatic carcinoma, and is expected to be applied to preparation of the drug preparation for treating the primary hepatic carcinoma by restraining the malignant cell proliferation of the primary hepatic carcinoma; and a new medicinal value is opened up for the fructus akebiae extract.

Description

technical field [0001] The present invention relates to the application of an extract of Semen Semen Semen in the treatment of primary liver cancer. Specifically, it relates to the application of the ethanol extract of Semen Semen Semen in the preparation of pharmaceutical preparations for the treatment of primary liver cancer, which belongs to the technical field of traditional Chinese medicine . Background technique [0002] Alias: August Zha, August Zha, August Hawthorn, August Melon, August Fruit, Hundred Days Melon, Cane Melon, Waxed Melon, Lala Fruit, Mutongzi, Yajingzi, Animal Weed, Na Akebia quinata (Thunb.) Decne., Akebia trifoliate (Thunb.) Koidz. ), Akebia trifoliate (Thunb.) Koidz.var.australis ((Diels)) Rehd.) dry near-ripe fruit. Seed of Prediction is bitter and cold in nature and taste, and returns to the liver, gallbladder, stomach, and bladder meridian; soothes the liver and regulates qi, harmonizes the stomach, promotes blood circulation and relieves pain...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P35/00
Inventor 方肇勤任红艳梁超张园园潘志强卢文丽刘小美
Owner SHANGHAI UNIV OF T C M
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