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Method for preparing hemin from yak blood powder as raw material

A technology of hemin and yak blood powder, which is applied in the preparation of hemin and the field of preparation of hemin, can solve the problems of increased industrial production costs, difficult transportation and storage of fresh blood, and achieves low cost and easy industrialization Production, easy-to-handle effects

Inactive Publication Date: 2013-07-10
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since fresh blood is not easy to transport and store, using liquid fresh blood as a raw material to extract and prepare hemin will bring a lot of inconvenience to industrial production and increase the cost of industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Accurately weigh the yak blood powder, add urea solution at a ratio of 6mol / L and 20mL to 2g blood powder, and ultrasonically treat for 30 minutes to fully dissolve; centrifuge the above solution at 3000r / min for 10 minutes, take the supernatant, and then filter to obtain the filtrate; Add 150mL of 3% acidic acetone to 150mL of the suction filtrate, stir and extract for 30min, then add 10mL of 3% sodium acetate, stir well, then adjust the pH to 7.0 with 1mol / L NaOH, centrifuge at 3000r / min for 10min, and use distilled water and absolute ethanol for precipitation Washing, and then drying to obtain hemin.

Embodiment 2

[0020] Accurately weigh yak blood powder, add urea solution at a ratio of 6mol / L and 25mL to 2g blood powder, and ultrasonically treat for 30min to fully dissolve; centrifuge the above solution at 3000r / min for 10min, take the supernatant, and then filter to obtain the filtrate; Add 150mL of 3% acidic acetone to 175mL of the suction filtrate, stir and extract for 30min, then add 15mL of 3% sodium acetate, stir evenly, then adjust the pH to 7.0 with 1mol / L NaOH, centrifuge at 3000r / min for 10min, and use distilled water and absolute ethanol for precipitation Washing, and then drying to obtain hemin.

Embodiment 3

[0022] Accurately weigh the yak blood powder, add urea solution at a ratio of 6mol / L and 30mL to 2g blood powder, and ultrasonically treat for 30min to fully dissolve it; centrifuge the above solution at 3000r / min for 10min, take the supernatant, and then filter to obtain the filtrate; Add 150mL of 3% acidic acetone to 200mL of the suction filtrate, stir and extract for 30min, then add 20mL of 3% sodium acetate, stir well, then adjust the pH to 7.0 with 1mol / L NaOH, centrifuge at 3000r / min for 10min, and precipitate with distilled water and anhydrous Washed with ethanol, and then dried to obtain hemin.

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PUM

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Abstract

The invention discloses a method for preparing hemin from yak blood powder as the raw material. The method of producing hemin comprises the following steps: yak blood powder dissolution, impurity removal, extraction and precipitation. The method for producing hemin is simple, easy to operate, low in cost and convenient for industrialized production. Moreover, with yak blood powder as the raw material for preparing hemin, the invention provides a novel way of development and application of yak blood powder.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for preparing hemin, in particular to a method for preparing hemin by using yak blood meal as a raw material. Background technique [0002] The yak is a unique and dominant livestock species on the Qinghai-Tibet Plateau. There are about 15 million yaks in the world. China is the main producer of yaks, accounting for about 92% of the world's total. At present, yak meat and milk are fully utilized, but yak blood has a strong fishy smell and poor palatability, so a large amount of blood is made into blood meal as a feed additive or directly discarded. The content of hemoglobin (Hb) in yak blood is very high, reaching 86.41±13.07g / L, and the content of whole blood iron is 28.95±4.37mmol / L, which is higher than that in pig blood. Therefore, yak blood is the best product for making heme It is a natural high-quality resource of its derivatives, which can produce huge econ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/22
Inventor 张卫兵高维东李晓鹏艾对元赵炜
Owner GANSU AGRI UNIV