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Rapid detection method for severe chylemia

A detection method and chyle blood technology, applied in the detection field, can solve the problems of blood scrap, increased control difficulty, and large human error, and achieve the effects of ensuring accuracy, improving utilization rate, and avoiding blood waste.

Inactive Publication Date: 2013-07-17
侯广安 +1
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AI Technical Summary

Problems solved by technology

Because chyle blood is mostly observed with the naked eye, human error is large, and the control difficulty increases. How to grasp the degree of chyle blood is different for each person, resulting in the scrapping of part of the blood, and it is easy to cause conflicts between blood stations and blood supply hospitals.

Method used

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Embodiment Construction

[0017] The severe chyle blood rapid detection method that the present embodiment relates to, the method comprises the following steps:

[0018] Step 1: Select the criteria for severe chylotemia:

[0019] a. Selection of normal plasma: take 2-3 equal parts of normal plasma without hemolysis, jaundice, and chyle and mix it in a clean glass vessel, take out 2ml into a plastic test tube as normal plasma;

[0020] b. Dilution of severe chyle blood: take 15-20 bags of chyle blood and number them, take the same number of plastic test tubes and correspond to the number of each chyle blood bag, take 1ml of chyle blood from each bag and put it in the corresponding plastic test tube, according to GB18469- According to the requirements in 2001, the chyle index > 5 is defined as severe chyle. Therefore, 4ml of normal saline is added to the plastic test tube to fully mix the chyle blood and normal saline at a ratio of 1:4 to achieve the purpose of dilution;

[0021] C. Determination of the...

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Abstract

The invention relates to a detection method and particularly relates to a rapid detection method for severe chylemia. The method comprises the following steps that: a severe chylemia standard is selected; the optical density (OD) value of the severe chylemia standard is detected to obtain a standard OD value; the OD value of to-be-detected blood plasma is detected to obtain a detected OD value; and the severe chylemia is judged. By adopting the technical scheme disclosed by the invention, the rapid quantitative detection for the severe chylemia is realized and the detection can be completed within several seconds on the premise that the work load of staff is not increased. In addition, the accuracy of the detection result of alanine aminotransferase (ALT) can be insured. More importantly, the blood waste caused by errors of human observation in the detection of the blood plasma is avoided and the utilization rate of blood is increased.

Description

[0001] technical field [0002] The invention relates to a detection method, in particular to a rapid detection method for severe chyle blood. Background technique [0003] In the existing blood supply process, there is mostly chyle blood. In simple terms, chyle blood is "blood with oily slick" or "lipid blood". The plasma is milky white or turbid. This kind of blood cannot be donated to Others have to pay special attention to whether they have abnormal blood lipids, such as hyperlipidemia. According to the statistics of the Blood Donation Center, the proportion of domestic blood donors with chyle blood is as high as 1%. Theoretically, transfusion of chyle blood to patients has no health hazards, but chyle blood contains a large amount of microparticles, which may cause microvascular blockage if it flows into the patient's body, so most hospital blood banks do not use it. According to the "Quality Requirements for Whole Blood and Component Blood" GB18469-2012, the appearan...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 侯广安孔卫建张媛高占海
Owner 侯广安
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