Preparation method of betulinic acid 28-O-beta-D-glucopyranoside and use of betulinic acid 28-O-beta-D-glucopyranoside

A technology of glucopyranoside and betulinic acid, applied in the field of biopharmaceuticals, can solve the problem of rare modification of betulinic acid, and achieve excellent anti-inflammatory effect

Inactive Publication Date: 2013-07-24
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, microbial glycosylation modifica

Method used

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  • Preparation method of betulinic acid 28-O-beta-D-glucopyranoside and use of betulinic acid 28-O-beta-D-glucopyranoside
  • Preparation method of betulinic acid 28-O-beta-D-glucopyranoside and use of betulinic acid 28-O-beta-D-glucopyranoside
  • Preparation method of betulinic acid 28-O-beta-D-glucopyranoside and use of betulinic acid 28-O-beta-D-glucopyranoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Preparation of betulinic acid 28-O-β-D-glucopyranoside:

[0058] Dissolve 200 mg of betulinic acid as a substrate in 20 mL of ethanol; prepare 100 mL of 0.5 g / mL glucose solution, and sterilize (conditions are the same as PDA); the strain NRRL1086 is inoculated into liquid PDA medium from a solid slope at 4°C (the medium is 100 mL 20% Potato decoction contains KH 2 PO 4 0.3 g; MgSO 4 0.75g; glucose 10g; vitamin B10.01g, the liquid medium was divided into 150mL Erlenmeyer flasks, each bottle containing 30mL), and cultured in a shaker for 24 hours (28°C, 180r / m), that is, the seeds liquid; the seed liquid was transferred to the liquid PDA medium (the composition of the medium was the same as before) under aseptic conditions, and continued to cultivate in the shaker for 24 hours. Add 1mL betulinic acid ethanol solution and 3mL sucrose solution to each bottle, and stop the reaction after culturing for 144 hours. The fermentation broth was extracted 6 times with an equal...

Embodiment 2

[0061] tablet

[0062] Take 100 g of betulinic acid 28-O-β-D-glucopyranoside prepared in Example 1, mix with 50 g of starch and 50 g of dextrin, use an appropriate amount of 30% ethanol as a wetting agent to make a soft material, and granulate by conventional methods , add an appropriate amount of magnesium stearate and mix to make a tablet.

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Abstract

The invention relates to the field of biological medicines and in particular relates to betulinic acid 28-O-beta-D-glucopyranoside (I). The invention discloses a preparation method of the betulinic acid 28-O-beta-D-glucopyranoside. The preparation method comprises the following step of carrying out biotransformation onto the betulinic by a bacterial strain with a preservation number of NRRL1086. The invention further discloses a use of the betulinic acid 28-O-beta-D-glucopyranoside in anti-inflammation.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a preparation method of betulinic acid 28-O-β-D-glucopyranoside, and the invention also discloses its anti-inflammatory application. Background technique [0002] Inflammation is one of the most common pathological processes in human diseases and is associated with most diseases. Anti-inflammatory drugs are the second largest class of drugs in clinical practice after anti-infective drugs. At present, the most commonly used anti-inflammatory drugs in clinical practice are mainly non-steroidal anti-inflammatory drugs and adrenocortical hormone drugs, but these drugs have multi-target effects. There are multiple ways, different doses and courses of treatment, and there are complex network relationships among various mechanisms. Long-term use will bring many adverse reactions. With the continuous development and understanding of traditional Chinese medicine and many years of clinic...

Claims

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Application Information

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IPC IPC(8): C12P33/20A61K31/7024A61P29/00
Inventor 余伯阳杜晨晖张剑许少华朱羽尧钱利武
Owner CHINA PHARM UNIV
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