Application of terfenadine in preparation of zebra fish heart function damage model

An injury model, terfenadine technology, applied in cardiovascular system diseases, active ingredients of heterocyclic compounds, drug combinations, etc., can solve the problem of not establishing a heart function injury model, and achieve lower production costs, stable and reliable repeatability, Create easy effects

Active Publication Date: 2015-07-15
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, unfortunately, a stable and recognized cardiac injury model has not yet been established in the zebrafish, a new model organism.

Method used

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  • Application of terfenadine in preparation of zebra fish heart function damage model
  • Application of terfenadine in preparation of zebra fish heart function damage model
  • Application of terfenadine in preparation of zebra fish heart function damage model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1. Establishment method of zebrafish cardiac function injury model

[0051] 1. Obtaining and using zebrafish larvae

[0052] For mating with wild-type zebrafish, a septum was used the day before pairing to ensure embryonic stage consistency. Use the same pair of embryos to conduct experiments, and the time of embryos born is controlled within half an hour to reduce the difference in development time between embryos. The embryos are stored in a petri dish with embryo culture medium, so that the embryos are not easy to mold, and also keep them in the water. The mineral content was the same, and they were put into an incubator at 28°C for two days. Add 0.2 mmol·L from zebrafish embryos 12 hours after birth -1 Phenylthiourea, replace the fresh drug solution once a day until two days after birth. The zebrafish two days after birth were placed under a dissecting microscope, and the zebrafish larvae with normal development were selected and placed in a 24-well plate...

Embodiment 2

[0065] Example 2. The protective effect of nicardil on zebrafish cardiac function damage caused by terfenadine

[0066] 1. Obtaining and using zebrafish larvae

[0067] For mating with wild-type zebrafish, a septum was used the day before pairing to ensure embryonic stage consistency. Use the same pair of embryos to conduct experiments, and the time of embryos born is controlled within half an hour to reduce the difference in development time between embryos. The embryos are stored in a petri dish with embryo culture medium, so that the embryos are not easy to mold, and also keep them in the water. The mineral content was the same, and they were put into an incubator at 28°C for two days. Add 0.2 mmol·L from zebrafish embryos 12 hours after birth -1 Phenylthiourea, replace the fresh drug solution once a day until two days after birth. The zebrafish two days after birth were placed under a dissecting microscope, and the zebrafish larvae with normal development were selected ...

Embodiment 3

[0080] Example 3. Protective effect of carvedilol on zebrafish cardiac function damage caused by terfenadine

[0081] 1. Obtaining and using zebrafish larvae

[0082] For mating with wild-type zebrafish, a septum was used the day before pairing to ensure embryonic stage consistency. Use the same pair of embryos to conduct the experiment, and the time of the embryos born is controlled within half an hour to reduce the difference in the development time of the embryos. The mineral content was the same, and they were put into an incubator at 28°C for two days. Add 0.2 mmol·L from zebrafish embryos 12 hours after birth -1 Phenylthiourea, replace the fresh drug solution once a day until 2 days after birth. The zebrafish two days after birth were placed under a dissecting microscope, and the zebrafish larvae with normal development were selected and placed in a 24-well plate, with 5 larvae in each well.

[0083] 2 compound treatment

[0084] Set up 6 experimental groups: 1 blan...

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Abstract

The invention relates to application of terfenadine in preparation of a zebra fish heart function damage model. The application comprises the following steps of putting normally developing zebra fish which is fertilized 2-3 days later into breeding water solution containing terfenadine, and cultivating for 24-48 hours at constant temperature of 28 DEG C to prepare the zebra fish heart function damage model. Terfenadine is utilized for the first time to build the zebra fish heart function damage model; the built zebra fish heart function damage model has the advantages that the zebra fish heart function damage model is simple and convenient to prepare, rapid, stable, reliable, and good in repeatability; the manufacturing cost of the heart function damage model is reduced; and the reliability of the experimental research result is improved.

Description

technical field [0001] The present invention relates to the application of terfenadine in preparing a zebrafish cardiac function injury model, in particular to the application of terfenadine in preparing a zebrafish cardiac function injury model for screening cardioprotective drugs, and belongs to the technical field of drug screening . Background technique [0002] Cardiac dysfunction is a syndrome of progressive cardiac dysfunction caused by various changes in the structure of the heart chambers or damage to the myocardium. Clinical manifestations include cardiac enlargement, arrhythmia, and heart failure. Cardiac dysfunction is a comprehensive reflection of the overall abnormal function of the heart. Viral infection, autoimmune reaction, heredity, drug poisoning and metabolic abnormalities can all cause cardiac dysfunction. The development of drugs capable of preventing and delaying the occurrence of cardiac function damage and / or alleviating the symptoms of clinical c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/445A61P9/00A61P9/06A61P9/04
Inventor 何秋霞刘可春韩利文彭维兵陈锡强张云王雪侯海荣王希敏楚杰
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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