Dry chemical test strip used in quantitative test of uric acid

A dry chemistry, test strip technology, applied in the field of biosensing, can solve problems such as affecting the accuracy of uric acid measurement

Inactive Publication Date: 2013-08-07
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since ascorbic acid in the blood easily reacts with hydrogen peroxi

Method used

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  • Dry chemical test strip used in quantitative test of uric acid
  • Dry chemical test strip used in quantitative test of uric acid

Examples

Experimental program
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Embodiment Construction

[0016] The following examples are intended to illustrate the present invention without further limiting the invention.

[0017] Dissolve 5g of dimethylferrocene in 15g of cyclohexanone solution and stir until fully dissolved, add 80g of carbon slurry and mechanically stir for 20min to mix well and set aside.

[0018] Cut the polyester film into a 90X300cm substrate in advance, wash it with secondary water, put it in an oven and dry it at 60°C for 15 minutes, and wait for cooling before use.

[0019] The prepared carbon paste was printed on a 250-mesh screen, and dried at 80°C for 30 minutes.

[0020] The Ag / AgCl paste was printed on a 250-mesh screen, and dried at 75°C for 15 minutes.

[0021] Print 10 g of ascorbate oxidase enzyme slurry containing 2ku on a 200-mesh water-based screen, and dry at 45°C for 15 minutes.

[0022] Paste the double-sided adhesive tape (5) and the hydrophilic film (6) on the siphon reaction area formed by the enzyme slurry printing area (4), and c...

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Abstract

The invention belongs to the technical field of biosensing, and relates to a dry chemical test strip used in quantitative test of uric acid and a preparation method thereof. The uric acid quantitative-test dry chemical test strip provided by the invention comprises an insulating substrate, electrodes and an anti-interference enzyme layer printed on the insulating substrate with a screen printing technology, and a siphonic reaction cell composed of a double-sided adhesive, a hydrophilic membrane, and the enzyme layer. According to the invention, with an electrochemical method, uric acid oxidation is catalyzed, and urate oxidase is not needed to participate in the reaction. Therefore, testing precision can be improved, cost can be reduced, and uric acid content can be tested in a short time.

Description

technical field [0001] The invention belongs to the technical field of biosensing, and relates to a dry chemical test strip for quantitatively testing uric acid and a preparation method thereof. Background technique [0002] Uric acid is the final product of purine metabolism in the human body and has no physiological function in the human body. Under normal circumstances, it is mainly excreted by the kidneys. The content of uric acid is the most commonly measured amount in clinical practice. The determination of serum uric acid content has important significance in the diagnosis of ventilation, acute cerebrovascular disease, heart failure disease, kidney disease and diabetes, so the quantitative determination of uric acid in serum is an important indicator of clinical detection. In addition, the determination of blood uric acid also has certain significance for the diagnosis and observation of leukemia, coronary heart disease, etc. It can be seen that the determination o...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 陈平彭勇贺敏闫新创张媛
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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