Method for induction and subculture maintenance on jasmine flower calluses
A callus induction and callus technology, applied in the field of plant tissue culture, can solve the problems of few literature and research, single flower color, shrinkage of jasmine cultivation area, etc., and achieve the effects of vigorous growth ability and high induction efficiency.
Active Publication Date: 2015-03-25
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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Problems solved by technology
However, with the decline of the jasmine tea market in recent years and the single flower color, the cultivation area of jasmine has shrunk greatly. For the sustainable development of the jasmine industry, it is necessary to vigorously develop the breeding of new varieties of jasmine, but traditional breeding methods have a great impact on jasmine. limitation
On the other hand, the research on jasmine is mainly concentrated on the previous researches such as cultivation and processing technology and analysis of physical and chemical properties. There are few reports on the biotechnology research of jasmine. Although the tissue culture of jasmine has been reported in recent years, the relevant literature and less research
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[0011] A method for Jasmine callus induction and subculture maintenance,
[0012] A. Selection and pretreatment of experimental materials
[0013] Cut the current year's branches or flower organs from the vigorously growing jasmine mother plant, remove the leaves, soak in water with detergent for 3-5 minutes, and then rinse with tap water for 1-2 hours; Branches are placed in an appropriate amount of 75% ethanol (v / v) and soaked for 30s, and then soaked with 0.1% HgCl 2 (w / v) Disinfect for 6-8 minutes (6 minutes for flower organs), then rinse with sterile water for 3-4 times. Under aseptic conditions, the stem segments or petals with a length of about 0.5 cm were cut out as explants for inoculation.
[0014] B. Initiate culture: get the Jasmine explants obtained, inoculate them into the induction medium, and place them in the culture room for cultivation. Callus formation can be seen after 7 days, and grow into primary callus in about 30 days; The conditions are temperature...
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The invention provides a method for induction and subculture maintenance on jasmine flower calluses. The method comprises the steps of treating jasmine flower explants, carrying out induction culture and carrying out multiplication culture and subculture maintenance. According to the method, not only are a large amount of the jasmine flower calluses quickly obtained but also the obtained jasmine flower calluses have the advantages of high induction efficiency and high quality, and meanwhile, the jasmine flower calluses can be subjected to subculture maintenance for a long term, namely the jasmine flowers have strong growth ability, so that a good foundation is laid for research on production of secondary metabolites by biotechnology and cell culture of the jasmine flowers and the like.
Description
technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing and maintaining jasmine callus. Background technique [0002] Jasmine is a subtropical evergreen shrub of the genus Frangipani (or Jasmine) in the family Oleaceae. The crown of jasmine is upright or semi-expanded, the corolla is white, very fragrant, and the flowering period is long; it is recorded in "Compendium of Materia Medica" that its flowers, leaves, and roots can be used as medicine. The leaves can reduce swelling and detoxify. Modern studies have also shown that jasmine root extract has anti-arrhythmic, sedative and hypnotic effects, inhibits the central nervous system, and reduces oxygen consumption. It has been widely used in ornamental potted plants, landscaping, scented tea cellar system, and spice production. Therefore, in my country, jasmine has penetrated into people's social life. It is an important garden flower with both orn...
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IPC IPC(8): A01H4/00
Inventor 赖呈纯刘波朱育菁甘煌灿陈文辉
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI