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Colloidal gold rapid diagnosis test paper of porcine 2 type torque teno virus antibody and preparation method thereof

A technique for rapid diagnosis of leukovirus, applied in the field of colloidal gold rapid diagnosis test paper for porcine type 2 leptovirus antibody and its preparation, achieving the effects of good sensitivity and specificity, convenient use, and simple structure

Inactive Publication Date: 2013-08-14
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no rapid diagnosis method for TTSuV2 antibody in the industry, so it is of great significance to provide a test strip for the rapid diagnosis method of TTSuV2 antibody

Method used

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  • Colloidal gold rapid diagnosis test paper of porcine 2 type torque teno virus antibody and preparation method thereof
  • Colloidal gold rapid diagnosis test paper of porcine 2 type torque teno virus antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] like figure 1 As shown, the colloidal gold rapid diagnostic test paper for porcine type 2 leukovirus antibody of the present invention comprises a PVC base plate 1, a sample pad 2, a colloidal gold binding pad 3, a nitrocellulose membrane 4 and a water-absorbing filter paper 5, and a sample pad 2 , colloidal gold binding pad 3, nitrocellulose membrane 4 and water-absorbing filter paper 5 are sequentially arranged on the PVC base plate 1 according to the chromatography direction, and one end of the sample pad 2 is stacked on the front end of the colloidal gold binding pad 3, and the back of the colloidal gold binding pad 3 The end is stacked on one end of the nitrocellulose membrane 4, and the front end of the water-absorbing filter paper 5 is stacked on the other end of the nitrocellulose membrane 4; the nitrocellulose membrane 4 is sprayed with a detection line 41 and a quality control line 42, respectively. The colloidal gold binding pad consists of a nitrocellulose m...

Embodiment 2

[0046] A colloidal gold rapid diagnostic test paper for porcine type 2 leukovirus antibody is prepared according to the same preparation method as in Example 1. The difference from Example 1 is that the pH value of the colloidal gold used in step 4) was adjusted to 10.0, and the mixing ratio of the dialyzed rTTSuV2 ORF1' protein solution and colloidal gold was 100 μL / 10mL.

Embodiment 3

[0048] A colloidal gold rapid diagnostic test paper for porcine type 2 leukovirus antibody is prepared according to the same preparation method as in Example 1. The difference from Example 1 is that the pH value of the colloidal gold used in step 4) was adjusted to 11.0, and the mixing ratio of the dialyzed rTTSuV2 ORF1' protein solution and colloidal gold was 150 μL / 10mL.

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Abstract

The invention discloses colloidal gold rapid diagnosis test paper of a porcine 2 type torque teno virus antibody and a preparation method thereof. The test paper comprises a PVC (Poly Vinyl Chloride) baseplate, a sample pad, a colloidal gold combination pad, a nitrocellulose membrane and water absorbing filter paper, wherein the sample pad, the colloidal gold combination pad, the nitrocellulose membrane and the water absorbing filter paper are sequentially arranged on the PVC baseplate in the chromatography direction; one end of the sample pad is folded at the front end of the colloidal gold combination pad; the back end of the colloidal gold combination pad is folded at one end of the nitrocellulose membrane; the front end of the water absorbing filter paper is folded at the other end of the nitrocellulose membrane; a detection line and a quality control line are respectively sprayed on the nitrocellulose membrane; and the colloidal gold combination pad is composed of a layer of nitrocellulose membrane and rTTSuV2ORF1' colloidal gold sprayed on the nitrocellulose membrane. The colloidal gold rapid diagnosis test paper of the porcine 2 type torque teno virus antibody, disclosed by the invention, is capable of rapidly detecting the TTSuV2 antibody and has the advantages of simple structure, convenience in use and good sensitivity and specificity.

Description

[0001] technical field [0002] The invention relates to the field of veterinary medicine, in particular to a colloidal gold rapid diagnostic test paper for porcine type 2 leukovirus antibody and a preparation method thereof. [0003] Background technique [0004] Transfusion transmitted virus (Transufsion Transmitted Virus, TTV) is a kind of non-enveloped, icosahedral, single-strand negative-strand circular DNA virus, and the diameter of virus particles is about 30-32 nm observed by electron microscope. TTV was first discovered by a Japanese scholar in the serum of a non-A-non-E post-transfusion hepatitis patient with an unknown pathogen, and then it was found in non-primates and various domestic and wild animals such as pigs, cattle, sheep, dogs, cats, and poultry. Infection with TTV has also been found in animals. In 2009, the International Society for Taxonomy of Viruses (ICTV) classified porcine TTV into the genus Iotatorquevirus of the family Anelloviridae, and fu...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/532
Inventor 李中圣刘小琴罗均赵焱陈轶雯
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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