Cytomechanics device capable of synchronously realizing flow load and fluorescence observation

Abstract

The invention discloses a cytomechanics device capable of synchronously realizing flow load and fluorescence observation. The device comprises a parallel flow cavity module used for simulating the hydrodynamic force environment of cells in a human body under a shear flow condition, an inverted fluorescence phase contrast microscope used for performing fluorescence excitation on the cells, a signal collecting module used for collecting weak fluorescence signals, and a control module used for synchronously triggering the parallel flow cavity module, the inverted fluorescence phase contrast microscope and the signal collecting module and analyzing and processing data. A special filter disc combination is selected to excite the cells of a sample plane on the inverted fluorescence phase contrast microscope, the parallel flow cavity module is used for applying mechanical stimulation on the cells, the control module is used for achieving synchronousness of mechanical stimulation, fluorescence excitation and collection, and an EMCCD (Electron Multiplying Chargecoupled Device) is used for sampling the data, reconstructing and analyzing the data of an image so as to realize the real-time dynamic combination of fluid dynamic load and fluorescence observation of population cells.

Description

technical field [0001] The invention relates to a cell mechanics device combining hydrodynamic loading and microfluorescence observation. Background technique [0002] The human body is always in a mechanical environment, and its biological processes are regulated by different mechanical environments, showing basic characteristics such as multiple factors, nonlinearity, and interaction. It is necessary to quantitatively understand its coupling laws at the micro level. Cells are not only in a complex biochemical environment, but also in different biomechanical environments; cell mechanics can clarify how cells sense, modify, and respond to the physical characteristics of the cell environment; cells communicate through chemical and physical signals Realize information exchange, and thus participate in a series of biological processes such as embryogenesis, wound healing, inflammatory response, tumor metastasis; the response of cells to mechanical stimuli is very important in i...

AI Technical Summary

Problems solved by technology

Mechanical loading experiment technology: applying mechanical effects to cells or molecules is to simulate the physiological and mechanical environment of cells or molecules, and it is impossible to realize the dynamic behavior of living cells (migration, proliferation, differentiation, etc.) and the cytoskeleton in living cells under mechanical stimulation , real-time dynamic detection of ionic and molecular activities and changes, and because cell culture, probe labeling and mechanical loading cannot be performed in situ, it is difficult to quantify test results and realize time-space coupling

Fluorescence detection and analysis imaging technology based on molecular optical labeling cannot achieve mechanical loading on molecules or cells, and it is also difficult to observe the coupling process of molecule-cell mechanics-chemistry and mechanobiology

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